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KCQS04 Sigma

KiCqStart® Probe qPCR ReadyMix

for Bio-Rad, Cepheid, Eppendorf, Illumina, Corbett, and Roche systems

Synonym: Kicqstart® Probe qPCR ReadyMix

Purchase

Properties

Related Categories Fast QPCR, KiCqStart qPCR ReadyMix, Molecular Biology, PCR/Amplification, Quantitative PCR,
form   liquid
feature   hotstart
storage condition   protect from light
color   light blue
shipped in   dry ice
storage temp.   −20°C

Description

Components

2X reaction buffer containing optimized concentrations of MgCl2, dNTPs (dATP, dCTP, dGTP, dTTP), hot-start DNA polymerase, insert blue qPCR dye, and stabilizers.

packaging:
250 reactions* = 2 X 1.25 mL tubes
1250 reactions* = 10 X 1.25 mL tubes
5000 reactions* = 1 X 50 mL tube
*number of reactions based on a 20uL volume

Features and Benefits

• Assay results in as little as 33 minutes
• Highly efficient and sensitive real-time PCR results
• Little/no optimization required

General description

KiCqStart Probe qPCR ReadyMix is an advanced qPCR reagent system for both fast and conventional PCR cycling protocols or instruments. It is a versatile and robust solution that provides the ultimate sensitivity and high PCR efficiency using a variety of fluorogenic probe chemistries, including TaqMan® hydrolysis probes. KiCqStart Probe qPCR ReadyMix is provided as a 2X concentrated ready-to-use reaction cocktail that contains all required reaction components, except primers, probe(s), and DNA template. The light blue color of an inert tracer dye simplifies reaction assembly in white, or clear, plates and helps to minimize pipetting or mixing errors. It does not interfere with qPCR performance or affect the stability of the product.

A key component of KiCqStart Probe qPCR ReadyMix is an ultra-pure, processive thermostable DNA polymerase that is free of detectable E. coli DNA§. KiCqStart Probe qPCR ReadyMix is ideal for demanding qPCR applications such as bacterial pathogen detection where residual host DNA in typical recombinant enzyme preparations can limit assay sensitivity and obscure detection of low copy samples. The enzyme in KiCqStart Probe qPCR ReadyMix is combined with high avidity monoclonal antibodies to provide a stringent automatic hot-start that allows reaction assembly, and temporary storage, at room temperature prior to PCR amplification.

Application

Different real-time PCR systems employ different strategies for normalization of fluorescent signals and correction of well-to-well optical variations. It is critical to match the appropriate qPCR reagent to your specific instrument.KiCqStart One-Step Probe RT-qPCR ReadyMix does not contain an internal reference dye. Compatible instruments include:Different real-time PCR systems employ different strategies for normalization of fluorescent signals and correction of well-to-well optical variations. It is critical to match the appropriate qPCR reagent to your specific instrument.KiCqStart One-Step Probe RT-qPCR ReadyMix does not contain an internal reference dye. Compatible instruments include:
• Bio-Rad® CFX384
• Bio-Rad CFX96
• Bio-Rad MiniOpticon
• Bio-Rad/MJ Chromo4
• Bio-Rad/MJ Opticon 2
• Bio-Rad/MJ Opticon
• Cepheid SmartCycler®
• Eppendorf Mastercycler® ep realplex
• Eppendorf Mastercycler ep realplex2 s
• Illumina Eco qPCR
• Qiagen/Corbett Rotor-Gene®3000
• Qiagen/Corbett Rotor-Gene 6000
• Qiagen/Corbett Rotor-Gene Q
• Roche LightCycler® 480

Legal Information

Bio-Rad is a registered trademark of Bio-Rad Laboratories, Inc.

CFX384 is a trademark of Bio-Rad Laboratories, Inc.

CFX96 is a trademark of Bio-Rad Laboratories, Inc.

Chromo4 is a trademark of Bio-Rad Laboratories, Inc.

KiCqStart is a registered trademark used under license.

LightCycler is a registered trademark of Roche

Mastercycler is a registered trademark of Eppendorf AG

MiniOpticon is a trademark of Bio-Rad Laboratories, Inc.

Opticon is a trademark of Bio-Rad Laboratories, Inc.

ReadyMix is a trademark of Sigma-Aldrich Co. LLC

Rotor-Gene is a registered trademark of Qiagen Group

Scorpions is a registered trademark of Qiagen Group

SmartCycler is a registered trademark of Cepheid, Inc.

TaqMan is a registered trademark of Roche Molecular Systems, Inc.

Price and Availability


NAP and qPCR Reagents from Roche

KiCqStart Probe Assays

Kapa Biosystems
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Protocols & Articles

Articles

Assay Optimization & Validation - PCR Technologies Guide

Assay optimization and validation are essential, even when using assays that have been predesigned and commercially obtained. Optimization is required to ensure that the assay is as sensitive as is r...
Keywords: AGE, Amplification, Buffers, Detection methods, Digestions, Electrophoresis, Gas chromatography, Gel electrophoresis, Gene expression, Melting, Nucleic acid annealing, Nucleic acid hybridization, Polymerase chain reaction, Polymerase chain reaction - quantitative, Polymerization reactions, Polymorphisms, Purification, RNA purification, Separation, Size-exclusion chromatography, Transcription

Data Analysis - PCR Technologies Guide

After a traditional PCR has been completed, the data are analyzed by resolution through an agarose gel or, more recently, through a capillary electrophoresis system. For some applications, a qPCR wil...
Keywords: Amplification, Cancer, Capillary electrophoresis, Cell culture, Degradations, Detection methods, Electrophoresis, Gene expression, Growth factors, Indicators, Microarray Analysis, Polymerase chain reaction, Polymerase chain reaction - quantitative, Purification, RNA purification, Sample preparations, Transcription

Polymerase Chain Reaction - PCR Technologies Guide

The polymerase chain reaction (PCR)1,2,3 has become one of the most widely used techniques in molecular biology. It is used in applications from basic research to high-throughput screening. While it ...
Keywords: Amplification, Cloning, Degradations, Detection methods, Electrophoresis, Evaporation, Gas chromatography, Gel electrophoresis, Indicators, Melting, Molecular biology, Nucleic acid annealing, Nucleic acid denaturation, Polymerase chain reaction, Polymerase chain reaction - quantitative, Purification, Sequencing, Size-exclusion chromatography, Transcription

Quantitative PCR - PCR Technologies Guide

As described in Introduction and Historical Timelines and Polymerase Chain Reaction, PCR is currently considered to be the most useful technique that is applied to investigations using molecular biol...
Keywords: Amplification, Buffers, Clinical, Clinical Chemistry, Detection methods, Electrophoresis, Enzyme activity, Gel electrophoresis, Gene expression, Melting, Methylations, Molecular biology, Nucleic acid annealing, Nucleic acid denaturation, Nucleic acid hybridization, Peptide synthesis, Polymerase chain reaction, Polymerase chain reaction - quantitative, Polymerization reactions, Purification, Size-exclusion chromatography, Transcription

Protocols

KiCqStart™ Probe qPCR ReadyMix™

KiCqStart Probe qPCR ReadyMix is an advanced qPCR reagent system for both fast and conventional PCR cycling protocols or instruments. It is a versatile and robust solution that provides the ultimate ...
Keywords: Amplification, Diagnostic, Nucleic acid annealing, Nucleic acid denaturation, Nucleic acid hybridization, Phase transitions, Polymerase chain reaction, Polymerase chain reaction - quantitative, Titrations

Related Content

PCR Selection Guide

Sigma-Aldrich offers a wide variety of PCR reagents to meet any experimental needs. Our range of polymerases is customized to meet your End-Point PCR, qPCR, or RT-PCR needs. Our products vary from ro...
Keywords: Polymerase chain reaction, Polymerase chain reaction - quantitative

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Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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