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KiCqStart® Probe Assays

Predesigned probe assays for gene expression analysis

Synonym: 5’ nuclease assay, Hydrolysis Probes, Predesigned Probes, Probe-based Gene Expression, Probe-based RT-qPCR

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Properties

storage temp.   −20°C

Description

Application

Product use: gene expression analysis

Features and Benefits

KiCqStart® Probe Assays are sensitive, specific, and will save you time and money by eliminating the tedious steps required to develop an effective probe-based RT-qPCR Assay. The probe is labeled with Sigma’s dark Onyx Quencher (OQ), which emits heat instead of light and therefore offers an improved signal-to-noise ratio compared to traditional fluorescent quenchers.

Benefits of these assays include:
• Target analysis is done
• Design characteristics are set
• Oligo characteristics are set
• Easy to order

General description

Overview
Predesigned KiCqStart Probe Assays are for quantifying gene expression by RT-qPCR (Reverse Transcription Quantitative Real-Time PCR). To comply with MIQE, they have been developed using sophisticated bioinformatics tools and validated in silico to avoid off-target amplification1. KiCqStart Probe Assays are available as up to three sets of a forward primer, a reverse primer, and a Dual-Labeled Probe for genes from common model organisms.

1SNP loci have been avoided during the design process when possible. Please consult dbSNP to determine if your gene has unavoidable SNPs.

To learn more about KiCqStart Probe Assays, visit our Frequently Asked Questions section.

Specifications
Design characteristics
• GC Content: 20 - 80%2
• Melting Temperature: 58.0 °C2
• Secondary Structure: None
• Excessive 3′ Primer Clamping: Minimal
• Amplicon size: 75 - 150 bases
• Splice variant detected: Most prevalent

Oligo characteristics (primers)
• Molecule: DNA (all sequences verified by mass spec QC)
• Internal Modifications: None
• Contents: 1 forward and 1 reverse primer
• Length: 18 - 27 bases2
• Purification: RP cartridge
• Format: 3 OD minimum yield, dry; 2 tubes with 1 oligonucleotide each

Oligo characteristics (probe)
• Molecule: DNA (all sequences verified by mass spec QC)
• Internal Modifications: None
• 5’ Reporter Dye: 6-FAM or HEX
• 3’ Quencher: Onyx Quencher A (OQA)
• Contents: 1 Dual-Labeled Probe
• Length: 21 - 27 bases2
• Purification: HPLC
• Format: 1 OD minimum yield, dry; 1 tube with 1 oligonucleotide

Sequences are provided at the time of shipment.

295% of primer pairs fall within these specifications

Physical properties

Properties include:
• Available species: human, mouse, and rat
• Components: three oligos, one per tube
• Shipping condition: dry, ambient temperature
• Storage condition: dry or wet, single-use aliquots

Legal Information

FAM is a trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries

HEX is a trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries

KiCqStart is a registered trademark used under license.

OQ is a trademark of Sigma-Aldrich Co. LLC

Onyx Quencher is a trademark of Sigma-Aldrich Co. LLC

Other Notes

Frequently Asked Questions section is available for this product.

Price and Availability

Ordering predesigned KiCqStart Probe Assays is easy using our state-of-the-art gene search tool. Open the tool, enter your gene, select your species, choose your assay, and add to cart.


Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles
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