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L0377 Sigma

L-Lactic Dehydrogenase from bovine heart

Type IX, Predominantly the LDH H4 isotype, ammonium sulfate suspension, ≥300 units/mg protein

Synonym: (S)-Lactate: NAD+ oxidoreductase, L-LDH, LAD, LD

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Properties

Related Categories 1.1.x.x Acting on hydroxyl groups, 1.x.x.x Oxidoreductases, Biochemicals and Reagents, Enzyme Class Index, Enzymes, Inhibitors, and Substrates More...
type   Type IX
form   ammonium sulfate suspension
foreign activity   glutamic-pyruvic transaminase <0.02%
  pyruvate kinase, myokinase, glutamic-oxalacetic transaminase and α-glycerophosphate dehydrogenase <0.01%
storage temp.   2-8°C
Gene Information   cow ... LDHA(281274), LDHB(281275)

Description

Analysis Note

Protein determined by biuret

Biochem/physiol Actions

Also catalyzes the oxidation of other L-2-hydroxymonocarboxylic acids.

Unit Definition

One unit will reduce 1.0 μmole of pyruvate to L-lactate per min at pH 7.5 at 37 °C.

Physical form

Crystalline suspension in 2.1 M (NH4)2SO4 solution, pH 6.0

Price and Availability

Safety & Documentation

Safety Information

WGK Germany 
3
Protocols & Articles

Protocols

Enzymatic Assay of L-Lactic Dehydrogenase (EC 1.1.1.27)

To standardize a procedure for the assay of L-Lactic Dehydrogenase for all sources from heart muscle.
Keywords: Extinction coefficient

Related Content

Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Molecular biology

Peer-Reviewed Papers
15

References

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L2625 L-Lactic Dehydrogenase from bovine heart, Type III, ammonium sulfate suspension, ≥500 units/mg protein

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