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L0651 Sigma

Lectin from Lycopersicon esculentum (tomato)

biotin conjugate, lyophilized powder

Synonym: Lycopersicon esculentum agglutinin, LEA

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Properties

Related Categories Lycopersicon esculentum (Tomato), Glycan Recognizing Proteins, Glycobiology, Lectins, Molecular Biology,
form   lyophilized powder
composition   Lectin, ≥20%
extent of labeling   ≥3 mol biotin per mol lectin
conjugate   biotin conjugate
storage temp.   −20°C

Description

Analysis Note

Agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 7.3, of a 1 mg/ml solution. This activity is the lowest concentration to agglutinate a 2% suspension of human erythrocytes after 1 hour incubation at 25 °C.

Biochem/physiol Actions

LEA is not blood group specific, but has an affinity for N-acetyl-β-D-glucosamine oligomers. The lectin is a glycoprotein containing approx. equal amounts of protein and carbohydrate and is reported to inhibit the mitogenic activity of phytohemagglutinin from Phaseolus vulgaris. This lectin binds endothelial cells and is used to visualize the microvasculature.

Physical form

Contains NaCl

Price and Availability


Amplified Detection

Amicon Pro Purification System
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
nwg

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles
Peer-Reviewed Papers
15

References

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