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  • L0663 - Anti-Laminin-2 (α-2 Chain) antibody, Rat monoclonal

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L0663 Sigma

Anti-Laminin-2 (α-2 Chain) antibody, Rat monoclonal

clone 4H8-2, purified from hybridoma cell culture

Synonym: Anti-Merosin

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies to Extracellular Matrix, Antibodies to Extracellular Matrix Proteins,
species reactivity   mouse, human
application(s)   immunocytochemistry: suitable
  immunohistochemistry (frozen sections): suitable
  immunoprecipitation: suitable
  indirect ELISA: suitable
  indirect immunofluorescence: 4-8 μg/mL using acetone-fixed frozen sections of human tongue
  microarray: suitable
  western blot: suitable
clone   4H8-2, monoclonal
concentration   ~2 mg/mL
antibody form   purified immunoglobulin
form   buffered aqueous solution
isotype   IgG1
mol wt   antigen mol wt ~400 kDa (denatured)
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... LAMA2(3908)
mouse ... Lama2(16773)
biological source   rat
conjugate   unconjugated

Description

Immunogen

mouse heart laminin-2

General description

The gene LAMA2 (laminin-2) encodes a protein belonging to a large family of heterotrimeric glycoproteins called laminins. It contains a large globular C-terminal domain that binds to dystroglycan and integrin α7β1 cell receptor specific for myofibers. The amino terminal domain associates with other laminins during the formation of protein meshwork that is involved in the basal membrane supramolecular assembly. The gene is mapped to human chromosome 6q22.33.

Physical form

Solution in phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Specificity

The epitope recognized by the antibody resides in the N-terminal portion of the α2 chain of laminin.

Preparation Note

Purified from the culture supernatant of hybridoma cells grown in a bioreactor.

Application

Anti-Laminin-2 (α-2 Chain) antibody, Rat monoclonal has been used in immunofluorescence and immunohistochemistry.

Biochem/physiol Actions

Laminins are glycoproteins that function in extracellular matrix architecture, cell adhesion, differentiation, and neurite outgrowth. Mutations in the gene LAMA2 (laminin-2) leading to either partial deficiency or complete absence of laminin α2 have been associated with limb-girdle muscular dystrophy (LGMD), characterized by severe, early-onset congenital muscular dystrophy to mild, later childhood-onset limb-girdle type muscular dystrophy, hypotonia with muscle weakness at early infancy, poor spontaneous movements with contractures of the large joints, and weak cry along with respiratory dysfunction.

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Safety Information

RIDADR  NONH for all modes of transport
WGK Germany  2
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Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Molecular biology, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers
15

References

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