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L1034 Sigma

Lectin from Helix pomatia

FITC conjugate, buffered aqueous solution

Synonym: Helix pomatia agglutinin, HPA

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Properties

Related Categories Helix pomatia, Biochemicals and Reagents, Fluorescent Conjugates, Fluorescent Probes, Labels, Particles and Stains, Glycan Recognizing Proteins,
form   buffered aqueous solution
potency   <1 μg per mL agglutination activity
composition   Protein, ≥0.5 mg/mL
extent of labeling   2-5 mol FITC per mol protein
conjugate   FITC conjugate
storage temp.   −20°C

Description

Analysis Note

Where reported, agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, of a 1 mg/ml solution. This activity is the lowest concentration to agglutinate a 2% suspension of human blood group A erythrocytes after 1 hour incubation at 25 °C.

Biochem/physiol Actions

HPA has anti-A human blood group specificity and has an affinity for terminal N-acetyl-α-D-galactosaminyl residues. Conjugates are prepared from affinity purified lectin.

Physical form

Solution in 0.01 M Tris, pH 8.0, containing 0.15 M NaCl and 0.1% sodium azide

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