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L1667 Sigma

Lysozyme human

recombinant, expressed in rice, ≥100,000 units/mg protein, lyophilized powder

Synonym: Lysobac r Lysozyme, Mucopeptide N-acetylmuramoylhydrolase, Muramidase

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Description

Features and Benefits

Lysobac is a breakthrough for bacterial cell lysis and can be used in diagnostic applications, bioprocessing and life science research. It is recombinant human lysozyme produced in an animal-free production system. Animal-free production eliminates the safety risk and inconsistent lot-to-lot performance of hen egg white lysozyme. Lysobac has significantly higher bioactivity than hen egg white lysozyme. One gram replaces 4 grams of hen egg white lysozyme. It also delivers gentle cell lysis, unlike mechanical lysis which can cause protein shear and reduce protein yields by up to 20%.
Advantages:
• Improves Bacterial Fermentation Efficiency
• Greater bacterial lysis activity per mg.
• 1 gram of delivers more lysis activity than 4 grams of HEWL
• Animal-free and consistent leading to improved regulatory efficiency for drug manufacturing
• Improved recombinant protein yield compared to mechanical lysis
• Reduces cost of goods

Other Notes

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

Packaging

1, 10 g in glass bottle

Package size based on protein content

Unit Definition

One unit will produce a ΔA450 of 0.001 per min at pH 6.24 at 25 °C, using a suspension of Micrococcus lysodeikticus as substrate, in a 2.6 mL reaction mixture (1 cm light path).

Legal Information

Lysobac is a trademark of InVitria

Price and Availability


Biomedical Applications
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Protocols

Enzymatic Assay of Lysozyme (EC 3.2.1.17)

This procedure may be used for Lysozyme products. The enzymatic rate determination (A450, Light path = 1 cm) is based on the following reaction:

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Peer-Reviewed Papers
15

References

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