Synonym: Phaseolus vulgaris agglutinin, PHA
Popular Documents: Specification Sheet (PDF)
|Related Categories||Phaseolus vulgaris (Red kidney bean), Bioactive Small Molecules, Cell Biology, Glycan Recognizing Proteins, Glycobiology,|
|form||lyophilized powder (contains ~50% buffer salts as sodium phosphate and NaCl)|
|potency||≤10 μg per mL mitogenic activity (Bromdeoxyuridine incorporation in human peripheral blood mononuclear cells)|
|color||white to off-white|
|solubility||PBS: soluble5 mg/mL, clear to slightly hazy, colorless to faintly yellow (pH 6.8)|
|suitability||suitable for cell culture|
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Potency and solubility expressed in mg lectin/ml
PHA consists of two molecular species, an erythroagglutinin (PHA-E) which has low mitogenic activity and high erythroagglutinin activity, and leucoagglutinin (PHA-L) which has high mitogenic and leucoagglutinating activity, but very low erythroagglutinating activity.1,2
PHA-E is not blood group specific, but agglutination can be inhibited by certain oligosaccharides.3 PHA-P is the protein form of PHA prior to separation and purification of erythroagglutinin and leucoagglutinin.4 PHA-M is the mucoprotein form.4 Conjugates are prepared from the corresponding purified lectins.
Sold on the basis of lectin content
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