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L2018 Sigma

Lipopolysaccharides from Escherichia coli K-235

purified by gel-filtration chromatography

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Description

Packaging

5, 25 mg in glass bottle

Preparation Note

The product is soluble in water (5 mg/ml) or cell culture medium (1 mg/ml) yielding a hazy, faint yellow solution. A more concentrated, though still hazy, solution (20 mg/ml) has been achieved in aqueous saline after vortexing and warming to 70-80 oC. Lipopolysaccharides are molecules that form micelles in every solvent. Hazy solutions are observed in water and phosphate buffered saline. Organic solvents do not give clearer solutions. Methanol yields a turbid suspension with floaters, while water yields a homogeneously hazy solution.

Biochem/physiol Actions

Lipopolysaccharides (LPS) are localized in the outer layer of the membrane and are, in noncapsulated strains, exposed on the cell surface. They contribute to the integrity of the outer membrane, and protect the cell against the action of bile salts and lipophilic antibiotics.

General description

This product is extracted from E. coli serotype K235 and purified by gel filtration. The source strain is ATCC 13027.

Price and Availability


Detect Your Unseen Protein
Safety & Documentation

Safety Information

Symbol 
GHS07  GHS07
Signal word 
Warning
Hazard statements 
RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Articles

Lipopolysaccharides

Lipopolysaccharide (LPS) is the major component of the outer membrane of Gram-negative bacteria. Lipopolysaccharide is localized in the outer layer of the membrane and is, in noncapsulated strains, e...
Glycobiology Analysis Manual, 2nd Edition
Keywords: Antibiotics, Asymmetric synthesis, Biochemistry, Cell disruption, Chemical biology, Chromatography, Clinical, Electrophoresis, Filtration, Gel electrophoresis, Gene expression, Immunology, Ion Exchange, Metabolism, Microbiology, Nuclear magnetic resonance spectroscopy, PAGE, Purification, Size-exclusion chromatography, Spectroscopy, Transduction, Veterinary

Protocols

Lipopolysaccharides

Lipopolysaccharides (LPS) are characteristic components of the cell wall of Gram negative bacteria; they are not found in Gram positive bacteria. They are localized in the outer layer of the membrane...
Keywords: Adsorption, Antibiotics, Cell culture, Cell disruption, Chromatography, Culture media, Filtration, Immunology, Ion Exchange, Metabolism, Positron Emission Tomography, Purification, Size-exclusion chromatography, Solvents

Peer-Reviewed Papers
15

References

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