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L2130 Sigma

Lactoperoxidase from bovine milk

ammonium sulfate suspension, ≥50 units/mg protein

Synonym: Donor:hydrogen peroxide oxidoreductase, Peroxidase

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Properties

Related Categories 1.11.x.x Peroxide as receptor, 1.x.x.x Oxidoreductases, Biochemicals and Reagents, Cell Biology, Enzyme Class Index,
form   ammonium sulfate suspension
absorbance ratio   A412/280 nm 0.60-0.85
storage temp.   2-8°C
Gene Information   cow ... LPO(280844)

Description

Analysis Note

Protein determined by Lowry method.

Biochem/physiol Actions

Lactoperoxidase catalyzes the oxidation of iodide to iodine by hydrogen peroxide. This activity provides a gentle, specific alternative to chloramine T for the radioiodination of proteins and DNA.

Other Notes

Using 2,2′-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) diammonium salt as substrate, two or three times the activity is observed.

Unit Definition

One unit will form 1.0 mg of purpurogallin from pyrogallol in 20 sec at pH 6.0 at 20 °C.

Physical form

Suspension in 3.2 M (NH4)2SO4 pH approx. 7.0

Price and Availability

Suggested Laboratory Gloves


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Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Articles

Ammonium Sulfate Suspensions - Technical Note

Note that for an ammonium sulfate suspension, most of the enzyme will be in solid form. It is likely that only negligible amounts of enzyme will be in the ammonium sulfate solution.
Keywords: Enzyme activity

Related Content

Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Molecular biology

Peer-Reviewed Papers
15

References

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