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L2157 Sigma

Laccase from Rhus vernicifera Green Alternative

crude acetone powder, ≥50 units/mg solid

Synonym: Benzenediol:oxygen oxidoreductase

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Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

General description

Sigma Life Science is committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for waste prevention when used in fuel cell and cellulosic ethanol research. For more information see the article in biofiles.

Packaging

10000 units in poly bottle

Unit Definition

One unit will produce a ΔA530 of 0.001 per min at pH 6.5 at 30 °C in a 3 mL reaction volume using syringaldazine as substrate.

Price and Availability


All labs need water

Biomedical Applications
Safety & Documentation

Safety Information

Symbol 
GHS08  GHS08
Signal word 
Danger
Hazard statements 
Precautionary statements 
RIDADR 
NONH for all modes of transport
WGK Germany 
1

Documents

Certificate of Analysis

Certificate of Origin

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis. These documents are located on the product detail page under Useful Links & Tools. Click on the following link to search for a Certificate of Analysis. Please click the following link to see the details on our Product Dating Information.
How do I get lot-specific information or a Certificate of Analysis?
A Certificate of Analysis is available by lot number and can be obtained through our Advanced Search Option: http://www.sigmaaldrich.com/catalog/AdvancedSearchPage.do
How do I find price and availability?
There are several ways to find pricing and availability for our products.  Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers. 
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS. To access the shipping information for this material, use the link on the product detail page for the product, or search here. 
How stable are solutions of Product L2157, Laccase from Rhus vernicifera?
Solutions should be prepared fresh, directly before use, as they are not stable as stock solutions.
My solution of Product L2157, Laccase from Rhus vernicifera, isn't clear. Is this normal?
Yes. L2157 is a crude acetone powder, and can contain other proteins, enzymes, and cellular debris.  A suspension may be prepared in 100 mM potassium phosphate buffer, pH 6.5, by homogenization with a hand-held homogenizer for 10 minutes. This preparation may then be centrifuged; the laccase activity will be present in the supernatant.
What is the molecular weight (MW) of Product L2157, Laccase from Rhus vernicifera?
Spira-Solomon and Solomon, J. Am. Chem. Soc., 109, 6421-6432 (1987) reports estimated molecular weights of 52 kDa (laccase 1), 55 kDa (laccase 2), and 70 kDa (laccase 3), as determined by gel filtration.
What is the pH optimum of Product L2157, Laccase from Rhus vernicifera?
Our own assay is performed at pH 6.5.  One publication (Casella et al., J. Inorg. Biochem., 100, 2127-2139 (2006)) gives pH 6.5 as another value for the laccase from this species, with a range for enzyme activity of 4.3 to 9.
What is the temperature optimum of Product L2157, Laccase from Rhus vernicifera?
Our own assay is performed at 30°C.  One publication (Casella et al., J. Inorg. Biochem., 100, 2127-2139 (2006)) gives 25°C as another value for the same enzyme from the same species.
My question is not addressed here, how can I contact Technical Service for assistance?
Use the option to the right to "Ask a Question" by email of a Technical Service Scientist.
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Protocols & Articles

Protocols

Enzymatic Assay of Laccase (EC 1.10.3.2)

To standardize a procedure for the enzymatic assay of laccase at Sigma-Aldrich, St. Louis, Mo.

Related Content

Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Molecular biology

Peer-Reviewed Papers
15

References

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