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L2164 Sigma

Anti-Luciferase antibody, Mouse monoclonal

clone LUC-1, purified from hybridoma cell culture

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Properties

Related Categories Alphabetical Index, Analytical and Industrial Enzymes, Antibodies, Biochemicals and Reagents, Enzymes, Inhibitors, and Substrates,
biological source   mouse
antibody form   purified immunoglobulin
clone   LUC-1, monoclonal
form   buffered aqueous solution
mol wt   mol wt 60 kDa
concentration   ~2 mg/mL
application(s)   immunocytochemistry: 20-40 μg/mL using transfected 293Tcells expressing luciferase
  immunocytochemistry: suitable
  western blot: 2-4 μg/mL using whole extract of transfected 293T cells expressing luciferase
  western blot: suitable
conjugate   unconjugated
shipped in   dry ice
storage temp.   −20°C

Description

Application

Antibody suitable for immunoblotting and immunocytochemistry.

Preparation Note

Working Dilution: 1-2 μg/ml

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

General description

The mouse monoclonal Anti-Luciferase antibody recognizes the recombinant luciferase in transfected cells. This antibody provides user with an alternative detection method for luciferase. Since it directly detects the luciferase protein, it is not dependent on either the luciferase activity or the measeurement of rapid reaction kinetics. This also allows for the detection of the luciferase enzyme expression in situ with reproducibility between sample sets.

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Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
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Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Molecular biology, Phosphorylations, Purification, Western blot

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