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L2254 Sigma

Lipoprotein Lipase from bovine milk

ammonium sulfate suspension, ≥2,000 units/mg protein (BCA)

Synonym: Diacylglycerol acylhydrolase, Diacylglycerol lipase

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Description

Packaging

5000 units in glass bottle

Preparation Note

Affinity purified

Unit Definition

One unit will release 1.0 nmole of p-nitrophenol per min at pH 7.2 at 37 °C using p-nitrophenyl butyrate as substrate.

Physical form

Suspension in 3.8 M ammonium sulfate, 0.02 M Tris HCl, pH 8.0

Price and Availability


All labs need water
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles

Articles

Ammonium Sulfate Suspensions - Technical Note

Note that for an ammonium sulfate suspension, most of the enzyme will be in solid form. It is likely that only negligible amounts of enzyme will be in the ammonium sulfate solution.
Keywords: Enzyme activity

Lipid Induced Insulin Resistance

Obesity is a well-established risk factor for the development of insulin resistance. Obesity is associated with the increased deposition of lipids in non-adipose tissue with subsequent decreases in i...
Linda Stephenson, Ph.D.
Biofiles v6 n4, 9
Keywords: Angiogenesis, Antivirals, Apoptosis, Biological processes, Cardiovascular, Catalog, Catalysis, Cell culture, Cell proliferation, Deposition, Diabetes, Events, Gene expression, Growth factors, Hormones, Inflammation, Metabolism, Metabolites, Obesity, Phosphorylations, Physiological Processes, Reductions, Transcription, Transduction, Type

Omega-3 Fatty Acids and Heart Disease

The potential for the prevention and treatment of cardiovascular disease through increased dietary intake of omega-3 (w-3) fish oils is not a recent scientific discovery. A historically important stu...
BioFiles 2007, 2.2, 14.
Keywords: Anti-inflammatory agents, Biochemistry, Cardiovascular, Clinical, Degradations, Esterifications, Immunology, Inflammation, Lipid Metabolism, Metabolism, Metabolites, Methods, Nutrition, Oxidations, Transcription

Protocols

LPL Activity Assay Protocol

The kit is sufficient for 100 assays in 200 µL total assay volume. Substrate Emulsion                                    0.125 mL Catalog Number MAK109A LPL Standard, 75.7 mM                         ...
Keywords: Sample preparations

Related Content

Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Molecular biology

Peer-Reviewed Papers
15

References

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