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L2263 Sigma

Lectin from Limulus polyphemus (horseshoe crab)

lyophilized powder

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Properties

form   lyophilized powder
potency   <16 μg per mL agglutination activity (with blood group B erythrocytes)
storage temp.   −20°C

Description

Analysis Note

Agglutination activity is expressed in μg per mL and is determined from serial dilutions in phosphate buffered saline, pH 7.4, containing 0.1 mM CaCl2, of a 1 mg per mL solution. This activity is the lowest concentration to agglutinate a 2% suspension of human blood group B erythrocytes. Bacterial agglutination is determined from serial dilutions of a 1 mg/ml solution in 0.01 M Tris, 0.15 M NaCl, pH 7.4, 0.01M CaCl2 with 1 mg/ml albumin and 0.92% TWEEN®.

Biochem/physiol Actions

Agglutinates Staphylococcus aureus cells, as well as human and horse erythrocytes.
Major affinity is for N-acetylated D-hexosamines, but is reported to have anti-galactan specificity.1

Packaging

Package size based on protein content

Preparation Note

Purified by affinity chromatography.

Physical form

Lyophilized powder containing Tris succinate, NaCl and calcium acetate

Price and Availability

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Protocols & Articles
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