L -Lactic Dehydrogenase from rabbit muscle
Type II, ammonium sulfate suspension, 800-1,200 units/mg protein
DOWNLOAD MSDS (PDF)Synonym:
(S)-Lactate: NAD+ oxidoreductase,
-
CAS Number 9001-60-9
-
EC Number 232-617-8
-
MDL number MFCD00131466
-
Popular Documents: Specification Sheet (PDF)
| Properties
| Related Categories | 1.1.x.x Acting on hydroxyl groups, 1.x.x.x Oxidoreductases, Application Index, Biochemicals and Reagents, Diagnostic and Analytical Enzymes, |
| type | Type II |
| form | ammonium sulfate suspension |
| foreign activity | pyruvate kinase, myokinase, malic dehydrogenase, glutamic-pyruvic transaminase, glutamic-oxalacetic transaminase and α-glycerophosphate dehydrogenase ≤0.01% |
| storage temp. | 2-8°C |
Description
Analysis Note
Protein determined by biuret.
Biochem/physiol Actions
Also catalyzes the oxidation of other
Unit Definition
One unit will reduce 1.0 μmole of pyruvate to
Physical form
Crystalline suspension in 3.2 M (NH4)2SO4 solution, pH 6.0
General description
Lactic Dehydrogenase (LDH) has a total molecular weight of 140 kDa and is composed of 4 subunits which are designated M subunit (muscle) and H subunit (heart). These subunits may be mixed in any of 5 combinations (M4, M3H1, M2H2, MH3, and H4). Skeletal muscle contains LDH that is predominately M4 with some small amounts of M3H and traces of H2H2. The H and M subunits are quite similar in molecular weight, but differ substantially in amino acid composition. Rabbit muscle LDH dissociates into dimeric species (MW = ~70 kDa) in acetate-chloride at pH 5.0, the dissociation is reversible. Biochemistry, 13, 3527-3531 (1974). Oxidizes glyoxylate and lactate.
Isoelectric point: 8.4-8.6
Optimal pH : 7.5 .
Price and Availability
Customers Also Viewed
Type III, ammonium sulfate suspension, ≥500 units/mg protein
Type XI, lyophilized powder, 600-1,200 units/mg protein
Type II, ammonium sulfate suspension, 350-600 units/mg protein
buffered aqueous glycerol solution, 900-1400 units/mL lactic dehydrogenase, 600-1,000 units/mL pyruvate kinase
Technical Service:
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Bulk Ordering & Pricing:
Need larger quantities for your development, manufacturing or research applications?
