• USA Home
  • L2524 - Lyticase from Arthrobacter luteus

EMAIL THIS PAGE TO A FRIEND
L2524 Sigma

Lyticase from Arthrobacter luteus

lyophilized powder, ≥2,000 units/mg protein, Protein ≥20 % by biuret

Purchase

Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Biochem/physiol Actions

Lyticase hydrolyzes poly-β(1→3)-glucose such as yeast cell wall glucan.

Other Notes

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

Packaging

10000, 25000 units in glass bottle

50000, 200000 units in poly bottle

Unit Definition

One unit will produce a ΔA800 of 0.001 per min at pH 7.5 at 25 °C, using a suspension of yeast as substrate in a 3 mL reaction mixture.

Physical form

Partially purified, lyophilized powder containing potassium phosphate buffer salts and stabilizers

Application

Yeast cells are difficult to disrupt because the cell walls may form capsules or resistant spores. DNA can be extracted from yeast by using lysing enzymes such as lyticase, chitinase, zymolase, and gluculase to induce partial spheroplast formation; spheroplasts are subsequently lysed to release DNA. Lyticase is preferred to digest cell walls of yeast and generate spheroplasts from fungi for transformation.
Reported to be useful for lysis of Ashbya, Candida, Debaryomyces, Eremothecium, Endomyces, Hansenula, Hanseniaspora, Kloeckera, Kluyveromyces, Lipomyces, Metschikowia, Pichia, Pullularia, Torulopsis, Saccharomyces, Saccharomycopsis, Saccharomycodes, and Schwanniomyces species.

Price and Availability


Available in ELITE Grade

All labs need water
Safety & Documentation

Safety Information

Symbol 
GHS08  GHS08
Signal word 
Danger
Hazard statements 
Precautionary statements 
RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles

Protocols

Enzymatic Assay of Lyticase

This procedure may be used for the determination of Lyticase activity using Baker’s yeast as the substrate. The turbidimetric rate determination [Absorbance at 800 nm (A800), Light path = 1 cm] is ba...
Keywords: Enzyme activity, Sonication

Peer-Reviewed Papers
15

References

Set your institution to view full text papers.
Related Products

related product

Product #

Description

Add to Cart

92210 Timestrip Plus 0 °C
06693 Timestrip Plus -20 °C

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?