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L2524 Sigma

Lyticase from Arthrobacter luteus

lyophilized powder, ≥2,000 units/mg protein, Protein ≥20 % by biuret

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Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Biochem/physiol Actions

Lyticase hydrolyzes poly-β(1→3)-glucose such as yeast cell wall glucan.

Other Notes

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

Packaging

10000, 25000 units in glass bottle

50000, 200000 units in poly bottle

Unit Definition

One unit will produce a ΔA800 of 0.001 per min at pH 7.5 at 25 °C, using a suspension of yeast as substrate in a 3 mL reaction mixture.

Physical form

Partially purified, lyophilized powder containing potassium phosphate buffer salts and stabilizers

Application

Yeast cells are difficult to disrupt because the cell walls may form capsules or resistant spores. DNA can be extracted from yeast by using lysing enzymes such as lyticase, chitinase, zymolase, and gluculase to induce partial spheroplast formation; spheroplasts are subsequently lysed to release DNA. Lyticase is preferred to digest cell walls of yeast and generate spheroplasts from fungi for transformation.
Reported to be useful for lysis of Ashbya, Candida, Debaryomyces, Eremothecium, Endomyces, Hansenula, Hanseniaspora, Kloeckera, Kluyveromyces, Lipomyces, Metschikowia, Pichia, Pullularia, Torulopsis, Saccharomyces, Saccharomycopsis, Saccharomycodes, and Schwanniomyces species.

Price and Availability


Ultrafree Centrifugal Filter Units

All labs need water

Biomedical Applications
Safety & Documentation

Safety Information

Symbol 
GHS08  GHS08
Signal word 
Danger
Hazard statements 
Precautionary statements 
RIDADR 
NONH for all modes of transport
WGK Germany 
3

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis. These documents are located on the product detail page under Useful Links & Tools. Click on the following link to search for a Certificate of Analysis. Please click the following link to see the details on our Product Dating Information.
How do I get lot-specific information or a Certificate of Analysis?
A Certificate of Analysis is available by lot number and can be obtained through our Advanced Search Option: http://www.sigmaaldrich.com/catalog/AdvancedSearchPage.do
How do I solubilize Product L2524, Lyticase?
The quality control enzymatic assay protocol for Lyticase uses cold deionized water to make a solution at 500 units/mL.
How can I store Product L2524, Lyticase, once reconstituted?
When lyticase is reconstituted and stored, it may be stable for no more than 4-6 hours before serious loss of activity is observed. It is suggested that fresh solutions be prepared for use.
How can Product L2524, Lyticase, be used to make yeast spheroplasts?
The use of lyticase in combination with chitinase and glucuronidase in the preparation of spheroplasts from C. albicans has been described in the following reference:  Calvert, C.M., and Sanders, D., "Inositol Trisphosphate-dependent and -independent Ca2+ Mobilization Pathways at the Vacuolar Membrane of Candida albicans."  J. Biol. Chem.  270, 7272-7280 (1995).
How do I find price and availability?
There are several ways to find pricing and availability for our products.  Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers. 
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS. To access the shipping information for this material, use the link on the product detail page for the product, or search here. 
My question is not addressed here, how can I contact Technical Service for assistance?
Use the option to the right to "Ask a Question" by email of a Technical Service Scientist.
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Protocols & Articles

Protocols

Enzymatic Assay of Lyticase

This procedure may be used for the determination of Lyticase activity using Baker’s yeast as the substrate. The turbidimetric rate determination [Absorbance at 800 nm (A800), Light path = 1 cm] is ba...
Keywords: Enzyme activity, Sonication

Related Content

Enzyme Explorer Key Resources: Lysing Enzymes

Cell Lysis Enzymes – How to tear down the Wall Enzymes provide a non-mechanical method for cell lysis and protoplast preparation. It may seem like a simple process to throw in your enzyme, stick your...
Keywords: Amplification, Biochemistry, Cell culture, Cell disruption, Culture media, Digestions, Enzymology, Metabolites, Molecular biology, PAGE, Purification, Sequencing

Peer-Reviewed Papers
15

References

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