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L2633 Sigma

Lectin from Ricinus communis (castor bean) Toxin RCA60–Peroxidase labeled

lyophilized powder

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Properties

form   lyophilized powder
composition   Protein, ~75% modified Warburg-Christian
storage temp.   −20°C

Description

Analysis Note

Agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 7.2, of a 1 mg protein per mL solution. This activity is the lowest concentration to agglutinate a 2% suspension of human erythrocytes after 1 hour incubation at 25°C.

Biochem/physiol Actions

Ricinus communis agglutinin (RCA) occurs in two forms designated RCA60 and RCA120 according to their mol. wt. of approx. 60,000 and 120,000 respectively.1 Neither RCA60 nor RCA120 is blood group specific. RCA60, also referred to as RCAII,2 Ricin D3 or RCL III4 is extremely toxic, inhibits protein synthesis and has an affinity for N-acetyl-D-galactosamine. RCA120, also referred to as RCAI2 or RCL (I + II)4 is an agglutinin and has an affinity for terminal β-D-galactosyl residues.1
Conjugates are prepared from affinity purified toxin, RCA60 or agglutinin RCA120.

Packaging

Package size based on protein content

Preparation Note

Prepared from peroxidase type VI using two step glutaraldehyde method of Avrameas, S. and Ternyck, T., Immunochemistry, 8, 1175 (1971).
Repurified after conjugation by affinity chromatography.

Unit Definition

One unit will form 1 mg purpurogallin in 20 sec from pyrogallol at pH 6.0 at 20°C.

Physical form

Lyophilized powder containing sodium citrate

Price and Availability

Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
Hazard statements 
Precautionary statements 
RIDADR 
UN 2811 6.1 / PGI
WGK Germany 
3

Documents

Certificate of Analysis

Protocols & Articles
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