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L2650 Sigma

Lectin from Glycine max (soybean)

peroxidase conjugate, lyophilized powder

Synonym: SBA, Soybean agglutinin

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Properties

Related Categories Glycine max (Soybean), Biochemicals and Reagents, Glycan Recognizing Proteins, Glycobiology, Lectins,
form   lyophilized powder
potency   <80 μg per mL agglutination activity
peroxidase activity   25-100 units/mg protein
composition   Protein, ~95% modified Warburg-Christian
conjugate   peroxidase conjugate
storage temp.   −20°C

Description

Analysis Note

Agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, of a 1 mg per mL solution. This activity is the lowest concentration to agglutinate a 2% suspension of human blood group A erythrocytes after 1 hr incubation at 25 °C.

Application

General Western Blot Protocol:
• Glycoprotein sample size: 500ng
• Lectin Concentration: 0.1ug/ml

  1. Load samples at 500 ng of glycoprotein per lane
    • Run 4-20% Bis-Tris SDS page gel
    • Transfer gel to a PVDF membrane
    • Block membrane for 1 hr at RT with RIPA buffer (R0278 Sigma)
    • Incubate HRP lectin at 0.1ug/ml with RIPA buffer for 2 hours at RT
    • Wash membrane 5 x 5 minutes with 25ml RIPA buffer
    • Detect using chemiluminescent substrate (CPS1-120)

Biochem/physiol Actions

SBA is not blood group specific but has an affinity for N-acetyl-D-galactosamine.

Preparation Note

Conjugates are prepared from affinity purified lectin.

Prepared from peroxidase type VI (P8375) using a modification of the method of O′Sullivan, which favors low molecular weight conjugates. Repurified after conjugation by affinity chromatography.

Unit Definition

One unit will form 1 mg purpurogallin in 20 sec from pyrogallol at pH 6.0, at 20 °C.

Physical form

Contains sodium citrate

Price and Availability


Amplified Detection

Amicon Pro Purification System
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Protocols & Articles
Peer-Reviewed Papers
15

References

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