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L2662 Sigma

Lectin from Viscum album (European mistletoe)

lyophilized powder

Synonym: Viscum album agglutinin, VAA

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Properties

Related Categories Viscum album (European mistletoe), Antitumor Agents, Cancer Research, Cell Biology, Glycan Recognizing Proteins,
form   lyophilized powder
potency   <16 μg/mL agglutination activity
composition   Protein, ~50% Lowry
storage temp.   −20°C

Description

Analysis Note

Agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, of a 1 mg/ml solution. This activity is the lowest concentration to agglutinate a 2% suspension of human blood group O erythrocytes after 1 hour incubation at 25 °C.

Biochem/physiol Actions

VAA is not blood group specific, but has an affinity for terminal β-D-galactosyl residues. VAA inhibits protein synthesis similarly to Ricin (RCA60) and inhibits allergen induced histamine release in vitro from human leukocytes.

Preparation Note

Purified by affinity chromatography using a modification of the method of Ziska.

Physical form

Highly purified, lyophilized powder containing phosphate buffer salts. Package size based on protein content.

Price and Availability


Amplified Detection

Amicon Pro Purification System
Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
Hazard statements 
Precautionary statements 
RIDADR 
UN 2811 6.1 / PGI
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles
Peer-Reviewed Papers
15

References

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