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L2785 Sigma

Lectin from Dolichos biflorus (horse gram)

lyophilized powder

Synonym: Dolichos biflorus agglutinin, DBA

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Properties

Related Categories Dolichos biflorus (Horse gram), Glycan Recognizing Proteins, Glycobiology, Lectins, Molecular Biology,
form   lyophilized powder
potency   ≤20 μg per mL agglutination activity
impurities   salt, essentially free
storage temp.   −20°C

Description

Analysis Note

Agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, of a 1 mg per mL solution. This activity is the lowest concentration to agglutinate a 2% suspension of human blood group A1 erythrocytes after 1 hour incubation at 25 °C.

Biochem/physiol Actions

DBA has anti-A1 human blood group specificity, useful for distinguishing between A1 and A2 blood types. DBA has strong anti-Cad activity, and has an affinity for terminal N-acetyl-α-D-galactosaminyl residues. Conjugates are prepared from affinity purified lectin.

Packaging

1, 5 mg in glass bottle

Preparation Note

Purified by affinity chromatography.

Price and Availability


Amplified Detection

Amicon Pro Purification System
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles
Peer-Reviewed Papers
15

References

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