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L2879 Sigma

Lysozyme chloride form from chicken egg white

Grade VI, ≥35,000 units/mg protein (E1%/282)

Synonym: Mucopeptide N-acetylmuramoylhydrolase, Muramidase

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Description

Application

Enzyme which breaks down the cell walls of bacteria; used to prepare spheroplasts.

Packaging

1, 5, 25 g in poly bottle

Preparation Note

3× Crystallized

Unit Definition

One unit will produce a ΔA450 of 0.001 per min at pH 6.24 at 25°C, using a suspension of Micrococcus lysodeikticus as substrate, in a 2.6 mL reaction mixture (1 cm light path).

Physical form

Lyophilized powder containing sodium chloride and sodium acetate

Price and Availability

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
2
Protocols & Articles

Protocols

Enzymatic Assay of Lysozyme (EC 3.2.1.17)

This procedure may be used for Lysozyme products. The enzymatic rate determination (A450, Light path = 1 cm) is based on the following reaction:

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Peer-Reviewed Papers
15

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