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L2884 Sigma

Leupeptin

microbial, ≥90% (HPLC)

Synonym: Acetyl-Leu-Leu-Arg-al, Leupeptin hemisulfate salt, N-Acetyl-L-leucyl-L-leucyl-L-argininal hemisulfate salt

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Properties

Related Categories Biochemicals and Reagents, Broad Spectrum Inhibitors of Proteolytic Enzyme Classes, Cell Biology, Cell Signaling and Neuroscience, Core Bioreagents,
Quality Level   PREMIUM
biological source   microbial
InChI Key   CIPMKIHUGVGQTG-VFFZMTJFSA-N
assay   ≥90% (HPLC)
solubility   H2O: 10 mM (Solutions are stable for a week at 4 °C. Stock solutions are stable up to 6 months at −20 °C.)
  H2O: 50 mg/mL
storage temp.   −20°C

Description

Analysis Note

Leupeptin gives multiple peaks on HPLC due to equilibria among three forms in solution. Purity determined using three main peaks. Majority of contaminating peptide is racemized leupeptin.

Packaging

5, 10, 25, 50, 100, 250 mg in glass bottle

Biochem/physiol Actions

Inhibitor of serine and cysteine proteases. Inhibits plasmin, trypsin, papain, calpain, and cathepsin B. Does not inhibit pepsin, cathepsins A and D, thrombin, or α-chymotrypsin. Effective concentration 10-100 μM. There have been numerous studies using leupeptin to protect against hearing loss caused by acoustic overstimulation or the ototoxic antibiotic gentamicin. (Loss of cochlear hair cells is believed to be mediated by calpain.)

Price and Availability


Ultrafree Centrifugal Filter Units

All labs need water

Biomedical Applications
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles

Articles

Roar Assay Kits for Studies in Lipoprotein Metabolism

This technical article is a compilation of select fluorescence-based activity assays developed by Roar Biomedical now offered through Sigma-Aldrich in kit format. The methods are well-characterized, ...
Robert Brocia
Roar Biomedical, Inc.
Keywords: Addition reactions, Cardiovascular, Chromatography, Clinical, Detection methods, Drug discovery, Esterifications, Gas chromatography, High performance liquid chromatography, Separation, Thin layer chromatography

Protocols

MTP Activity Assay Kit Protocol

The kit is sufficient for 100 assays in 200 µL total assay volume. Donor Particle                                0.4 mL     Catalog Number MAK110A Acceptor Particle                            0.4 mL ...
Keywords: Buffers, Homogenization, Sample preparations

Peer-Reviewed Papers
15

References

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92210 Timestrip Plus 0 °C
06693 Timestrip Plus -20 °C

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