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L2890 Sigma

Lectin from Helix pomatia

lyophilized powder

Synonym: Helix pomatia agglutinin, HPA

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Properties

Related Categories Helix pomatia, Glycan Recognizing Proteins, Glycobiology, Lectins, Molecular Biology,
form   lyophilized powder
packaging   vial of 5 mL
storage temp.   −20°C

Description

Analysis Note

Where reported, agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, of a 1 mg/ml solution. This activity is the lowest concentration to agglutinate a 2% suspension of human blood group A erythrocytes after 1 hour incubation at 25 °C.

Biochem/physiol Actions

HPA has anti-A human blood group specificity and has an affinity for terminal N-acetyl-α-D-galactosaminyl residues. Conjugates are prepared from affinity purified lectin.

Reconstitution

Reconstitute with 5.0 mL of 0.9% saline solution or 0.01 M phosphate buffered saline for a working strength reagent for determining human Type A1 blood in tube or tile tests.

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Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Protocols & Articles
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L3382 Lectin from Helix pomatia, lyophilized powder, salt, free

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