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L2895 Sigma

Lectin from Bandeiraea simplicifolia (Griffonia simplicifolia)

Isolectin B4 (BSI-B4), FITC conjugate, lyophilized powder

Synonym: Bandeirea simplicifolia agglutinin, BS-I

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Properties

Related Categories Bandeiraea simplicifolia (Griffonia simplicifolia), Cell Biology, Cell Signaling and Neuroscience, Glycan Recognizing Proteins, Glycobiology,
form   lyophilized powder
potency   <50 μg per mL agglutination activity (using human blood group B erythrocytes)
composition   Protein, ≥70% Lowry (balance sodium citrate)
extent of labeling   ≥1 mol FITC per mol lectin
conjugate   FITC conjugate
storage temp.   2-8°C

Description

Analysis Note

Agglutination activity is expressed in μg/mL and is determined from serial dilutions of a 1 mg/mL solution using phosphate buffered saline, pH 6.8, containing, for each lectin, calcium, magnesium, and manganese at different concentrations. This activity is the lowest concentration to agglutinate a 2% suspension of appropriate erythrocytes after 1 hr incubation at 25 °C.

Biochem/physiol Actions

BS-I has a major affinity for terminal α-D-galactosyl residues with a secondary affinity for terminal N-acetyl-α-D-galactosaminyl residues.

Other Notes

BS-I is a tetrameric lectin consisting of two types of subunits designated A and B. There are five BS-I isolectins with different subunit composition: BSI-B4, BSI-AB3, BSI-A2B2, BSI-A3B and BSI-A4. BSI-B4 is blood group B specific and has an exclusive affinity for terminal α-D-galactosyl residues, whereas BSI-A4 has blood group A specificity and has a major affinity for terminal N-acetyl-α-D-galactosaminyl residues.

Packaging

0.2, 1 mg in serum bottle

Package size based on protein content by Lowry.

Price and Availability


Amplified Detection

Amicon Pro Purification System
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles
Peer-Reviewed Papers
15

References

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