|Related Categories||A - M, All Solid Media/Agars, Broth / Media, Cloning, Core Bioreagents,|
|grade||for molecular biology|
|storage temp.||room temp|
|suitability||nonselective for Escherichia coli and Coliforms|
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5 g/L Yeast Extract
5 g/L NaCl
Lennox LB powder with agar provides:
• A budget-friendly alternative to pre-poured plates
• Easy scale-up using larger package sizes
• Standard formulation
Lennox LB is a highly-referenced microbial growth medium used for the cultivation of E. coli. This nutrient-rich microbial broth contains peptides, amino acids, water-soluble vitamins, and carbohydrates in a low-salt formulation. The addition of agar provides a solid medium for microbial growth.
Add 35g powder in 1L water. Heat to boiling while stirring to dissolve powder. Autoclave for 15 minutes at 121C to sterilize. Allow to cool slightly before making additions, such as antibiotics (if desired). Pour into petri dishes and allow to solidify.
Suitable for non-selective cultivation of E. coli strains for cloning, DNA plasmid production and production of recombinant proteins. Also suitable for selective cultivation when appropriate antibiotics are added, including those that require low-salt conditions, such as Zeocin®.
Zeocin is a registered trademark of Cayla Sarl
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