|Related Categories||A - M, All Liquid Media/Broths, Broth / Media, Cloning, Core Bioreagents,|
|grade||for molecular biology|
|pH range||6.8 - 7.2 (2% solution)|
|storage temp.||room temp|
|suitability||nonselective for Escherichia coli and Coliforms|
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5 g/L Yeast Extract
5 g/L NaCl
Lennox LB is a highly-referenced microbial growth medium used for the cultivation of E. coli. This nutrient-rich microbial broth contains peptides, amino acids, water-soluble vitamins, and carbohydrates in a low-salt formulation.
Stir to suspend 20g powder in 1L water. Autoclave for 15 minutes at 121C to sterilize. Allow to cool before making additions, such as antibiotics (if desired).
Lennox LB powder provides:
• Convenient small package to eliminate weighing
• Easy scale-up using larger package sizes
• A budget-friendly alternative to liquid
• Standard formulation
Zeocin is a registered trademark of Cayla Sarl
Microbiological studies require the availability of different species of bacteria, yeast or viruses in the laboratory. Large scale processes such as fermentation, protein and vaccine production requi...
Keywords: Antibiotics, Cellular processes, Cloning, Fermentation, Gene expression, Growth factors, Indicators, Molecular biology, Nutrition, Sterilizations, Vitamins, transformation
From our library of Protocols, Sigma-Aldrich presents Microbial Growth Protocols
Atlas, R. M., L.C. Parks Handbook of Microbiological Media, (1993)
The CprS sensor kinase of the zoonotic pathogen Campylobacter jejuni influences biofilm formation and is required for optimal chick colonization. Sarah L. Svensson et al Mol. Microbiol. 71, 253-72, (2009)
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