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L3022 Sigma

LB Broth (Lennox)

Powder microbial growth medium

Synonym: Luria Bertani Broth

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Description

Frequently Asked Questions

Live Chat and Frequently Asked Questions are available for this Product.

Components

10g/L Tryptone
5 g/L Yeast Extract
5 g/L NaCl

General description

Lennox LB is a highly-referenced microbial growth medium used for the cultivation of E. coli. This nutrient-rich microbial broth contains peptides, amino acids, water-soluble vitamins, and carbohydrates in a low-salt formulation.

Reconstitution

Stir to suspend 20g powder in 1L water. Autoclave for 15 minutes at 121C to sterilize. Allow to cool before making additions, such as antibiotics (if desired).

Features and Benefits

Lennox LB powder provides:
• Convenient small package to eliminate weighing
• Easy scale-up using larger package sizes
• A budget-friendly alternative to liquid
• Standard formulation

Legal Information

Zeocin is a registered trademark of Cayla Sarl

Price and Availability

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New Competent Cells

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Safety & Documentation

Safety Information

WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Articles

Introduction to Microbial Media

Microbiological studies require the availability of different species of bacteria, yeast or viruses in the laboratory. Large scale processes such as fermentation, protein and vaccine production requi...
Keywords: Antibiotics, Cellular processes, Cloning, Culture media, Fermentation, Gene expression, Growth factors, Indicators, Molecular biology, Nutrition, Sterilizations, Vitamins, transformation

Protocols

Microbial Growth Protocols

From our library of Protocols, Sigma-Aldrich presents Microbial Growth Protocols
Keywords: Antibiotics

Transforming E. coli with Engineered Plasmid

Inoue and colleagues developed this method in 1990. It works well for many strains commonly used in cloning. The original method calls for growing the overnight E. coli cultures at 18°C. We find that...
Keywords: Antibiotics, Centrifugation, Cloning, Peptide synthesis, Phase transitions, transformation

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