• USA Home
  • L3037 - Escort III Transfection Reagent

EMAIL THIS PAGE TO A FRIEND
L3037 Sigma

Escort III Transfection Reagent

Lipid reagent for transfecting sensitive and primary cells

Purchase

Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Components

Escort III formulation:
1 mg/mL total lipid in water

Note the identity of the lipids used in Escort III is confidential.

Application

Suitable for transient and stable transfection of nucleic acids into cultured eukaryotic cells. Use approximately 2-8 μl Escort III and 2 μg DNA per 6 cm cell culture plate. Protocol optimization provides very efficient transfection. The following cells have been successfully transfected using Escort III:

A549
C2C12 myotubes
Cardiomyocytes (rat)
COS-7
Fibroblasts (rat)
Germ cells (male rat)
Hepatocytes (rat and hamster)
HepG2
HeLa
Jurkat
Keratinocytes (human)
Myoblasts (mouse and quail)
Myocytes (mouse)
NIH3T3
PC-12
Retinal Neurons (rat)
Tracheobronchial cells (sheep)

Caution

Do not freeze.

Features and Benefits

• Suitable for stable and transient transfection
• Optimized for a wide variety of primary cells
• Low toxicity
• Compatible with both serum and serum-free transfection protocols
• Ideal for PC-12 cells

General description

Escort III is a unique formulation of a proprietary polycationic lipid and a neutral non-transfecting lipid. This liposome-forming compound is used for transfection of nucleic acids into primary cells.

Principle

A stable complex is formed when Escort III is mixed with DNA in the absence of serum. The complexes are stable and can be directly added to the cell culture medium, where they fuse with the cell membrane, releasing the DNA into the cytoplasm. Note: complex formation is inhibited by serum, but once stable complexes have formed, the presence of serum is without consequence.

Legal Information

Escort is a trademark of Sigma-Aldrich Co. LLC

Price and Availability

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Articles

Introduction to Transfection

Transfection involves the introduction of DNA or RNA into eukaryotic cells and is used in research to study and modulate gene expression. Thus, transfection techniques serve as an analytical tool tha...
Keywords: Apoptosis, Biological techniques, Drug discovery, Gene expression, Genetic, Precipitation, Transfection, transformation

Transfection Best Practices

The work of scientific research is an art which requires good attention to detail.  The quality of transfection is highest when these commonly used best practices and notes on optimization are employ...
Keywords: Antibiotics, Apoptosis, Growth factors, Molecular biology, Purification, Transfection

Transfection Reagent Selection Guide

Choosing a transfection reagent is easier when you can rely on the work of researchers who have used it before you. During development of each reagent, good transfection efficiency was verified in a ...
Keywords: Transfection

Protocols

Escort™ III Transfection Reagent Protocol

Escort III is a liposome suspension composed of a polycationic lipid and a neutral, non-transfecting lipid compound.  Transfection using liposomes is a commonly used method for the introduction of DN...
Keywords: Cell culture, Transfection

Escort™ IV Transfection Reagent Protocol

Escort IV is a liposome suspension composed of a polycationic lipid and a neutral, non-transfecting lipid compound.  Transfection using liposomes is a commonly used method for the introduction of DNA...
Keywords: Cell culture, Transfection

Lipid Transfection Optimization Protocol

There are many steps of the lipid transfection protocol that can be optimized if the initial transfection attempt is not successful. You may boost transfection efficiency and/or lower toxicity by opt...
Keywords: Cell culture, Transfection

Peer-Reviewed Papers
15

References

Set your institution to view full text papers.
Related Products

related product

Product #

Description

Add to Cart

XTG9-RO X-tremeGENE 9 DNA Transfection Reagent, Polymer reagent for transfecting common cell lines
XTGHP-RO X-tremeGENE HP DNA Transfection Reagent, High-performance polymer reagent for transfecting many cell lines
SITRAN-RO X-tremeGENE siRNA Transfection Reagent, Polymer reagent for delivering siRNA to common cell lines
L3287 Escort IV Transfection Reagent, Lipid reagent for transfecting mammalian and insect cells
T0956 Sigma Universal Transfection Reagent, Polymer reagent for transfecting common mammalian cell lines

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?