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L3126 Sigma

Lipase from porcine pancreas

Type II, 100-500 units/mg protein (using olive oil (30 min incubation)), 30-90 units/mg protein (using triacetin)

Synonym: PPL, Pancreatic lipase, Triacylglycerol acylhydrolase, Triacylglycerol lipase

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Description

Analysis Note

Protein determined by biuret.

Packaging

25, 100, 500 g in poly bottle

Quality

Crude (Steapsin). Contains amylase and protease activity.

Unit Definition

One unit will hydrolyze 1.0 microequivalent of fatty acid from triacetin in 1 hr at pH 7.4 at 37°C. (pH 7.7 is used with olive oil as substrate)

Application

Lipase from porcine pancreas has been used:
• for the analysis of fatty acid at the 2-position of triglycerides.
• to determine the indigestible fraction of plant source that is resistant to the action of digestive enzymes.
• as a component of dissolution media to study the dissolution profiles of theophylline.

Lipases are used industrially for the resolution of chiral compounds and the transesterification production of biodiesel.

Biochem/physiol Actions

Lipases catalyze the hydrolysis of esters in aqueous solutions and the synthesis of esters in non-aqueous solutions. They also produce hydroxyl and carboxylic groups through the hydrolysis of ester linkages in poly(ethylene terephthalate).

Lipases catalyze the hydrolysis of triacylglycerols into glycerol and free fatty acids.

Price and Availability


Available in ELITE Grade
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
1
RTECS 
TO9776500
Protocols & Articles

Articles

Lipid Induced Insulin Resistance

Obesity is a well-established risk factor for the development of insulin resistance. Obesity is associated with the increased deposition of lipids in non-adipose tissue with subsequent decreases in i...
Linda Stephenson, Ph.D.
Biofiles v6 n4, 9
Keywords: Angiogenesis, Antivirals, Apoptosis, Biological processes, Cardiovascular, Catalog, Catalysis, Cell culture, Cell proliferation, Deposition, Diabetes, Events, Gene expression, Growth factors, Hormones, Inflammation, Metabolism, Metabolites, Obesity, Phosphorylations, Physiological Processes, Reductions, Transcription, Transduction, Type

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Peer-Reviewed Papers
15

References

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