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L3160 Sigma

Monoclonal Anti-Leptin antibody produced in mouse

clone LEP-13, ascites fluid

Synonym: Anti-OB

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Obesity Research, Antibodies to Hormones,
species reactivity   human
application(s)   indirect ELISA: suitable
  radioimmunoassay: suitable
  western blot: 1:1,000 using recombinant human leptin
clone   LEP-13, monoclonal
antibody form   ascites fluid
isotype   IgM
mol wt   antigen mol wt 16 kDa
contains   15 mM sodium azide
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... LEP(3952)
biological source   mouse
conjugate   unconjugated

Description

Immunogen

recombinant human leptin.

General description

Leptin is a hormone secreted by adipocytes and regulates energy homeostasis, inflammation and neuroendocrine processes, synaptic formation and memory condensation. The physiological effects of leptin are mediated by leptin receptors, ObR and LepR. The receptors are present in brain, lungs, kidneys, liver and adipose tissue. Leptin is the key regulator of obesity and acts directly on neuronal networks to regulate feeding, metabolism, gastric mobility and energy homeostasis. The pathways that mediate the effect of leptin are JAK/STAT, PI3K and JNK. Deficiency of leptin may be a congenital condition and results in thyroid axis abnormalities.. Monoclonal Anti-Leptin reacts specifically with human leptin. A weak cross reactivity with mouse leptin is observed in radioimmunoassay.

Specificity

The antibody reacts specifically with human leptin. By RIA, weak cross-reactivity has been observed with recombinant mouse leptin.

Application

Monoclonal Anti-Leptin antibody may be used for immunoblotting at a working dilution of 1:1000. It has been used for detection by immunoblotting in human ovarian epithelial carcinoma cell line, OVCAR-3. The antibody is suitable for radioimmunoassay and ELISA.

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Safety Information

RIDADR  NONH for all modes of transport
WGK Germany  3
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Monoclonal and polyclonal primary antibodies are focused on cell biology, neurobiology and molecular biology. Secondary antibodies targeting multiple host’s IgG are conjugated to alkaline phosphatase...
Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Inflammation, Molecular biology, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers
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