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L3410 Sigma

Anti-Leptin antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

Synonym: Anti-OB

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Mesenchymal Stem Cells, Antibodies for Obesity Research,
species reactivity   mouse, human
application(s)   indirect ELISA: 1:1,000 using human or mouse recombinant leptin
  microarray: suitable
  western blot: 1:5,000 using human recombinant leptin
clone   polyclonal
antibody form   affinity isolated antibody
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... LEP(3952)
mouse ... Lep(16846)
biological source   rabbit
conjugate   unconjugated

Description

Immunogen

synthetic peptide corresponds to the amino terminal (amino acid residues 22-40) of human/mouse leptin.

Physical form

Solution in phosphate buffered saline containing 1% bovine serum albumin and 15 mM sodium azide.

Preparation Note

The antibody is affinity isolated on peptide-agarose.

Application

Anti-Leptin antibody produced in rabbit was used in western blotting of total protein extracted from intra-abdominal fat pad of mice.

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Biochem/physiol Actions

Leptin is a hormone secreted by adipocytes and regulates energy homeostasis, inflammation and neuroendocrine processes, synaptic formation and memory condensation. The physiological effects of leptin are mediated by leptin receptors, ObR and LepR. Leptin is the key regulator of obesity and acts directly on neuronal networks to regulate feeding, metabolism, gastric mobility and energy homeostasis. The pathways that mediate the effect of leptin are JAK/STAT, PI3K and JNK.

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Safety Information

RIDADR  NONH for all modes of transport
WGK Germany  3
Protocols & Articles

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Antibody Explorer | Buy Primary & Secondary Antibodies

Monoclonal and polyclonal primary antibodies are focused on cell biology, neurobiology and molecular biology. Secondary antibodies targeting multiple host’s IgG are conjugated to alkaline phosphatase...
Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Molecular biology, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers
15

References

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