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L3892 Sigma

Lectin from Triticum vulgaris (wheat)

peroxidase conjugate, lyophilized powder

Synonym: WGA, Wheat germ agglutinin

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Description

Application

Detects glycoproteins containing β(1→4)-N-acetyl-D-glucosamine when used with appropriate peroxidase substrate

General Western Blot Protocol:
• Glycoprotein sample size: 500ng
• Lectin Concentration: 0.1ug/ml

  1. Load samples at 500 ng of glycoprotein per lane
    • Run 4-20% Bis-Tris SDS page gel
    • Transfer gel to a PVDF membrane
    • Block membrane for 1 hr at RT with RIPA buffer (R0278 Sigma)
    • Incubate HRP lectin at 0.1ug/ml with RIPA buffer for 2 hours at RT
    • Wash membrane 5 x 5 minutes with 25ml RIPA buffer
    • Detect using chemiluminescent substrate (CPS1-120)

Biochem/physiol Actions

WGA is not blood group specific but has an affinity for N-acetyl-β-D-glucosaminyl residues and N-acetyl-β-D-glucosamine oligomers. WGA contains no protein-bound carbohydrate.

Packaging

1 mg in glass bottle

Package size based on protein content.

2 mg in serum bottle

Preparation Note

Conjugates are prepared from affinity purified lectin.

Prepared from peroxidase type VI (P8375) using a modification of the method of O′Sullivan, which favors low molecular weight conjugates. Repurified after conjugation by affinity chromatography.

Unit Definition

One unit will form 1.0 mg of purpurogallin from pyrogallol in 20 sec at pH 6.0 at 20 °C.

Physical form

Contains citrate buffer

Price and Availability


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Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles
Peer-Reviewed Papers
15

References

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