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L4022 Sigma

Ricin A chain from Ricinus communis (castor bean)

deglycosylated, buffered aqueous glycerol solution



Related Categories Ricinus communis (Castor bean), Cell Biology, Cell Signaling and Neuroscience, Glycan Recognizing Proteins, Glycobiology,
form   buffered aqueous glycerol solution
potency   ≥250 μg/mL per mL agglutination activity
composition   Protein, 0.5-3.0 mg/mL Lowry-TCA
storage temp.   2-8°C


Analysis Note

Agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 7.2, of a 1 mg/ml solution. This activity is the lowest concentration to agglutinate a 2% suspension of human erythrocytes after 1 hour incubation at 25°C.

Biochem/physiol Actions

Ricin A chain inactivates the 60 S ribosomal subunit of eukaryotic cells, inhibiting protein synthesis. This toxic activity is independent of the presence of the B chain of ricin.

The carbohydrate moiety of Ricin A chain has been modified using a metaperiodate-cyanoborohydride mixture. The product still inhibits protein synthesis in cell-free systems, and its removal from the blood stream by the liver is markedly decreased. Immunotoxins prepared from deglycosylated Ricin A chain will therefore be more effective in killing target cells.

Other Notes

Ricinus communis toxin (also named RCA60, RCAII, Ricin D, or RCL III) is a highly toxic protein, mol. wt. ~60 kDa, composed of two chains, A and B, connected by a single disulfide bridge.

Physical form

Solution in 40% glycerol containing 10 mM phosphate, pH 6.0, 0.15 M NaCl, 10 mM galactose and 0.5 mM dithioerythritol

Price and Availability

Amplified Detection

All labs need water
Safety & Documentation

Safety Information

GHS06  GHS06
Signal word 
Hazard statements 
Precautionary statements 
UN 3172 6.1 / PGIII
WGK Germany 
Protocols & Articles
Peer-Reviewed Papers


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