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L4144 Sigma

Lectin from Phaseolus vulgaris (red kidney bean)

Leucoagglutinin PHA-L, lyophilized powder, BioReagent, suitable for cell culture

Synonym: Phaseolus vulgaris agglutinin, PHA

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Properties

Related Categories Phaseolus vulgaris (Red kidney bean), Cell Biology, Cell Culture, Cell Signaling and Neuroscience, Glycan Recognizing Proteins,
product line   BioReagent
form   lyophilized powder
potency   erythroagglutination activity (Does not agglutinate a 2% suspension of human erythrocytes at 250 μg lectin/ml.)
  ≤1.0 μg per mL leucoagglutination activity
  ≤5 μg per mL mitogenic activity (Bromdeoxyuridine incorporation in human peripheral blood mononuclear cells)
impurities   salt, free
solubility   DPBS: soluble5 mg/mL, slightly hazy, colorless
suitability   leucocyte agglutination activity tested
  suitable for cell culture
storage temp.   2-8°C

Description

Analysis Note

Agglutination activity is expressed in μg/ml and is determined from serial dilutions of a 1 mg/ml solution. This activity is the lowest concentration to agglutinate a suspension of either human erythrocytes (2% in phosphate buffered saline, pH 6.8) or human leukocytes (107 per mL in saline) after 1 hr incubation at 25 °C.

Biochem/physiol Actions

PHA consists of two molecular species, an erythroagglutinin (PHA-E) which has low mitogenic activity and high erythroagglutinin activity, and leucoagglutinin (PHA-L) which has high mitogenic and leucoagglutinating activity, but very low erythroagglutinating activity.
PHA-E is not blood group specific, but agglutination can be inhibited by certain oligosaccharides. PHA-P is the protein form of PHA prior to separation and purification of erythroagglutinin and leucoagglutinin. PHA-M is the mucoprotein form. Conjugates are prepared from the corresponding purified lectins.

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Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
RTECS 
TJ3470000

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles
Peer-Reviewed Papers
15

References

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L8902 Lectin from Phaseolus vulgaris (red kidney bean), Phytohemagglutinin PHA-M, lyophilized powder, BioReagent, suitable for cell culture
08168 Timestrip Plus 8 °C

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