L4146 Sigma

Leptin human

>97% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder

Synonym: OB



Related Categories Cell Biology, Cell Signaling and Neuroscience, Cytokines Growth Factors and Hormones (Obesity), Cytokines, Growth Factors and Hormones, Gastrointestinal Peptides,
recombinant   expressed in E. coli
assay   >97% (SDS-PAGE)
form   lyophilized powder
mol wt   predicted mol wt ~16 kDa
storage temp.   −20°C
Gene Information   human ... LEP(3952)


Analysis Note

The biological activity is measured by its ability to induce proliferation of leptin-dependent human OB-R transfected murine BAF3 cells.

Physical form

Lyophilized from a 0.2 μm filtered solution in 10 mM Tris-HCl and 150 mM NaCl, pH 8.5.


Leptin human has been used:
• to study the effect of leptin on ACAT1 (acyl-coenzyme A:cholesterol acyltransferase 1) expression and cholesterol efflux from primary cultured human macrophages
• to study the effect of leptin on expression of IGFBP2 (insulin growth factor binding protein 2) in skeletal muscle
• to study the effect of leptin on HLA-G (human leukocyte antigen G) expression in placental trophoblasts
• to study the effect of leptin on in vitro maturation, fertilization and development of horse oocytes after ICSI (intracytoplasmic sperm injection)

Biochem/physiol Actions

Leptin is a hormone produced primarily in adipocytes, although leptin mRNA has also been identified in placenta and fetal tissues, gastric tissue and liver. Its primary site of action appears to be on neurons in the hypothalamus that are involved in regulating energy balance, appetite, and body weight. Leptin increases the production of nitric oxide in endothelial cells and stimulates angiogenesis in vitro and in vivo.
Human and mouse leptin share ~84% sequence identity.

Mutations in LEP (leptin) are linked with type-2 diabetes. Presence of high levels of leptin in plasma is related to adiposity.

General description

The gene LEP (leptin) is mapped to human chromosome 7q31. It is a 16kDa peptide. Leptin is released in the circulation and goes to the CNS (central nervous system). In CNS, it works by associating with leptin receptor.

Price and Availability

Amplified Detection

All labs need water
Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 


Certificate of Analysis

Certificate of Origin

Protocols & Articles


Lipid Induced Insulin Resistance

Obesity is a well-established risk factor for the development of insulin resistance. Obesity is associated with the increased deposition of lipids in non-adipose tissue with subsequent decreases in i...
Linda Stephenson, Ph.D.
Biofiles v6 n4, 9
Keywords: Angiogenesis, Antivirals, Apoptosis, Biological processes, Cardiovascular, Catalog, Catalysis, Cell culture, Cell proliferation, Deposition, Diabetes, Events, Gene expression, Growth factors, Hormones, Inflammation, Metabolism, Metabolites, Obesity, Phosphorylations, Physiological Processes, Reductions, Transcription, Transduction, Type

Peer-Reviewed Papers


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