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L4391 Sigma

Lipopolysaccharides from Escherichia coli O111:B4

γ-irradiated, BioXtra, suitable for cell culture

Synonym: LPS

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Properties

Related Categories Bacteria-derived adjuvants, Cell Biology, Cell Culture, Cell Signaling and Neuroscience, Immune Cell Signaling and Blood,
Quality Level   PREMIUM
sterility   γ-irradiated
product line   BioXtra
form   lyophilized powder
purified by   gel-filtration chromatography
storage condition   desiccated
impurities   <1% Protein (Lowry)
solubility   H2O: soluble5 mg/mL, hazy
suitability   suitable for cell culture
storage temp.   2-8°C

Description

Application

Lipopolysaccharides (LPSs) are characteristic components of the cell wall of Gram-negative bacteria. LPS and its lipid A moiety stimulate cells of the innate immune system by the Toll-like receptor 4 (TLR4), a member of the Toll-like receptor protein family, which recognizes common pathogen-associated molecular-patterns (PAMPs).

This LPS serotype has been used to stimulate B-cells and induce NOS in human hepatocytes.

Packaging

1 mg in glass bottle

Reconstitution

Lipopolysaccharides are supplied as lyophilized, γ-irradiated powders. To reconstitute, add 1 ml sterile balanced salt solution or tissue culture medium to the vial (1 mg) and gently swirl until the powder dissolves. Reconstituted product may be further diluted to desired working concentrations using sterile balanced salt solution or tissue culture medium.

Biochem/physiol Actions

LPS is a major constituent of the cell wall of most gram negative bacteria. It is a highly immunogenic antigen with the ability to enhance immune responses to soluble antigens. LPS also acts as a specific mitogen for bone marrow derived B lymphocytes from mice, rabbits, chickens, cows, hamsters, and humans.

Price and Availability


All labs need water
Safety & Documentation

Safety Information

Symbol 
GHS07  GHS07
Signal word 
Warning
Hazard statements 
RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles

Protocols

Lipopolysaccharides (LPS)

Lipopolysaccharides (LPS) are characteristic components of the cell wall of Gram negative bacteria; they are not found in Gram positive bacteria. They are localized in the outer layer of the membrane...
Keywords: Adsorption, Antibiotics, Cell culture, Cell disruption, Chromatography, Culture media, Filtration, Immunology, Ion Exchange, Metabolism, Positron Emission Tomography, Purification, Size-exclusion chromatography, Solvents

Peer-Reviewed Papers
15

References

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