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L4395 Sigma

Liposome Kit: Lipid mixtures for the preparation of liposomes

lyophilized powder

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Properties

Related Categories Biochemicals and Reagents, Lipid Kits, Lipids More...
form   lyophilized powder
composition   Cholesterol, 9 μmol/package
  L-α-Phosphatidylcholine (egg yolk), 63 μmol/package
  Stearylamine, 18 μmol/package
storage temp.   −20°C

Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Biochem/physiol Actions

Liposome Kit: Lipid mixtures for the preparation of liposomes contains cholesterol; L-α-phosphatidylcholine and stearylamine. Liposome kit may be used to prepare cationic (positively charge) liposomes for incorporation of materials into cells.

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Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
Hazard statements 
Target organs 
Gastrointestinal tract, Immune system, Liver
RIDADR 
NONH for all modes of transport
WGK Germany 
3

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis. These documents are located on the product detail page under Useful Links & Tools. Click on the following link to search for a Certificate of Analysis. Please click the following link to see the details on our Product Dating Information.
How do I get lot-specific information or a Certificate of Analysis?
A Certificate of Analysis is available by lot number and can be obtained through our Advanced Search Option: http://www.sigmaaldrich.com/catalog/AdvancedSearchPage.do
I have purchased Product L4395, Liposome Kit.  Is there a difference between positive and negative liposomes?
The choice is dependent upon what is being encapsulated and how it is being used.  A good reference discussing this question is Ogiso, T., et al., Effect of positively and negatively charged liposomes on skin permeation of drugs. J. Drug Target, 9(1), 49-59 (2001).
How is Product L4395, Liposome Kit, used?
The Liposomes of a large multilamellar structure type are the most widely used. They can be prepared using Sigma liposome mixtures by concentrating an organic solvent solution of lipids to a thin film on the walls of a container such as by rotary evaporation or by drying in glass with a stream of nitrogen gas. Then, under appropriate conditions, an aqueous solution is added to the thin film and with agitation multilamellar vesicles should form spontaneously with little effort. Large or small unilamellar structured liposomes can also be prepared by varying procedure or conditions. Please see the product insert for Product No. L4031 (the link below) for a step by step protocol that will work for this product also. http://www.sigmaaldrich.com/sigma/datasheet/l4031dat.pdf
How do I prepare the Product L4395, Liposome Kit liposomes?
Refer to the Product Information Sheet for L4031 for preparation information by clicking on the following link: http://www.sigmaaldrich.com/etc/medialib/docs/Sigma/Datasheet/4/l4031dat.Par.0001.File.tmp/l4031dat.pdf
I'm thinking of purchasing Product L4395, Liposome Kit.  Why would one use positive vs. negative liposomes?
Analogous to poly-L-lysine, one may need positively charged agents to deliver drugs to cells, hence positive liposomes.
How do I find price and availability?
There are several ways to find pricing and availability for our products.  Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers. 
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS. To access the shipping information for this material, use the link on the product detail page for the product, or search here. 
My question is not addressed here, how can I contact Technical Service for assistance?
Use the option to the right to "Ask a Question" by email of a Technical Service Scientist.
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Protocols & Articles
Peer-Reviewed Papers
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92210 Timestrip Plus 0 °C
06693 Timestrip Plus -20 °C

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