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L4516 Sigma

Lipopolysaccharides from Escherichia coli O127:B8

BioXtra, suitable for cell culture, γ-irradiated

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Properties

Related Categories Bacteria-derived adjuvants, Cell Biology, Cell Culture, Cell Signaling and Neuroscience, Immune Cell Signaling and Blood,
sterility   γ-irradiated
product line   BioXtra
form   lyophilized powder
purified by   gel-filtration chromatography
impurities   <1% Protein (Lowry)
solubility   H2O: soluble5 mg/mL, slightly hazy
suitability   suitable for cell culture
storage temp.   2-8°C

Description

Application

Lipopolysaccharides (LPSs) are characteristic components of the cell wall of Gram-negative bacteria. LPS and its lipid A moiety stimulate cells of the innate immune system by the Toll-like receptor 4 (TLR4), a member of the Toll-like receptor protein family, which recognizes common pathogen-associated molecular-patterns (PAMPs).

Lipopolysaccharides from Escherichia coli 0127:B8 has been used:
• to study the LPS-induced macrophage survival in mice
• to elicit the secretion of cytokines by cattle PBMC
• to study apoptosis of highly susceptible macrophage cells in a LPS-induced proinflammatory environment
• to perform autophagy assay in BV2 and BV2-LC3 cells
• for stimulation of human monocytes, macrophages and mouse microglia cells
• to induce pentraxin-3 expression in 293T cells

Reconstitution

Lipopolysaccharides are supplied as lyophilized, γ-irradiated powders. To reconstitute, add 1 ml sterile balanced salt solution or tissue culture medium to the vial (1 mg) and gently swirl until the powder dissolves. Reconstituted product may be further diluted to desired working concentrations using sterile balanced salt solution or tissue culture medium.

Biochem/physiol Actions

Lipopolysaccharides (LPSs) are characteristic components of the cell wall of Gram-negative bacteria. LPS and its lipid A moiety stimulate cells of the innate immune system by the Toll-like receptor 4 (TLR4). Being an immunogenic antigen, it enhances immune responses to soluble antigens.

Price and Availability


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Safety & Documentation

Safety Information

Symbol 
GHS07  GHS07
Signal word 
Warning
Hazard statements 
RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Protocols

Lipopolysaccharides

Lipopolysaccharides (LPS) are characteristic components of the cell wall of Gram negative bacteria; they are not found in Gram positive bacteria. They are localized in the outer layer of the membrane...
Keywords: Adsorption, Antibiotics, Cell culture, Cell disruption, Chromatography, Culture media, Filtration, Immunology, Ion Exchange, Metabolism, Positron Emission Tomography, Purification, Size-exclusion chromatography, Solvents

Peer-Reviewed Papers
15

References

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