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L4522 Sigma

Sodium dodecyl sulfate solution

for molecular biology, 10% in 18 megohm water

Synonym: Lauryl sulfate sodium salt, SDS, Sodium lauryl sulfate solution

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Properties

grade   for molecular biology
sterility   0.2 μm filtered
concentration   10% in 18 megohm water
foreign activity   DNase, RNase and NICKase, none detected

Description

Application

Useful in nucleic acid hybridization.

Price and Availability

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Safety & Documentation

Safety Information

Symbol 
GHS05  GHS05
Signal word 
Danger
Hazard statements 
Precautionary statements 
WGK Germany 
2

Protocols & Articles

Protocols

TRI Reagent® Protocol

1A. Tissue: Homogenize tissue samples in TRI Reagent (1 ml per 50–100 mg of tissue) in a Polytron® or other appropriate homogenizer.
Keywords: AGE, Buffers, Cell disruption, Centrifugation, Degradations, Digestions, Electrophoresis, Evaporation, Gel electrophoresis, Homogenization, PAGE, Polymerase chain reaction, Precipitation, Sample preparations, Separation, Solvents, Western blot

Peer-Reviewed Papers

References

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Chromatin isolation by RNA purification (ChIRP). Ci C Chu, Jeffrey J Quinn, Howard Y HY Chang J. Vis. Exp. 61, (2012)

Genomic maps of long noncoding RNA occupancy reveal principles of RNA-chromatin interactions. Ci C Chu, Kun K Qu, Franklin L FL Zhong, Steven E SE Artandi, Howard Y HY Chang Mol. Cell. 44, 667-78, (2011)

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Biofilm development and enhanced stress resistance of a model, mixed-species community biofilm. Lee KW, Periasamy S, Mukherjee M, et al. ISME J. 8(4), 894-907, (2014)

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Loss of mural cells leads to wall degeneration, aneurysm growth, and eventual rupture in a rat aneurysm model. Marbacher S, Marjamaa J, Bradacova K, et al. Stroke 45(1), 248-54, (2014)

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Straightforward palladium-mediated synthesis and biological evaluation of benzo[j]phenanthridine-7,12-diones as anti-tuberculosis agents. Davie Cappoen et al Eur. J. Med. Chem. 48, 57-68, (2012)

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Characterization of UDP-glucose dehydrogenase and UDP-glucose pyrophosphorylase mutants of Proteus mirabilis: defectiveness in polymyxin B resistance, swarming, and virulence. Sin-Sien Jiang et al Antimicrob. Agents Chemother. 54, 2000-9, (2010)

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Fate and effects of the surfactant sodium dodecyl sulfate. Singer MM and Tjeerdema RS Rev. Environ. Contam. Toxicol. 133, 95-149, (1993)

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[Complexes formed between sodium dodecyl sulfate and protein polypeptide with special reference to SDS-polyacrylamide gel electrophoresis (author's transl)]. Takagi T, Shirahama K, and Kubo K Tanpakushitsu Kakusan Koso. 21(10), 811-29, (1976)

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[Sodium dodecyl sulfate (SDS)--for the users in the field of biochemistry (author's transl)]. Tsujii K and Takagi T Tanpakushitsu Kakusan Koso. 19(9), 705-16, (1974)

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Effect of sodium dodecyl sulfate (SDS) on stress response in the Mediterranean mussel (Mytilus Galloprovincialis): regulatory volume decrease (Rvd) and modulation of biochemical markers related to oxidative stress. Messina CM, Faggio C, Laudicella VA, et al. Aquat. Toxicol. 157, 94-100, (2014)

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Influence of mounthrinse solutions on the color stability and microhardness of a composite resin. Lepri CP, Ribeiro MV, Dibb A, et al. Int. J. Esthet. Dent. 9(2), 238-46, (2014)

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Use of statistical modeling to predict the effect of formulation composition on conditioning shampoo performance. Lepilleur C, Giovannitti-Jensen A, and Kyer C J. Cosmet. Sci. 64(6), 411-27, (2013)

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[Antimicrobial activity of stable silver nanoparticles of a certain size]. Mukha IuP, Eremenko AM, Smirnova NP, et al. Prikl. Biokhim. Mikrobiol. 49(2), 215-23, (2013)

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Refolding of SDS-denatured proteins using amphipathic cosolvents and osmolytes. Roussel G, Tinti E, Perpète E, et al. Curr. Protoc. Protein Sci. Chapter 28, Unit28.5, (2013)

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"Mixed" anionic and non-ionic micellar liquid chromatography for high-speed radiometabolite analysis of positron emission tomography radioligands. Nakao R and Halldin C J. Chromatogr. A 1281, 54-9, (2013)

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Effect of curing light distance and different mouthwashes on the sorption and solubility of a nanofilled composite. de Azevedo Miranda D, dos Santos Bertoldo CE, Ambrosano GM, et al. Eur. J. Esthet. Dent. 8(1), 88-102, (2013)

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Capillary electrophoresis in presence of sodium dodecyl sulfate and a sieving medium. Takagi T Electrophoresis 18(12-13), 2239-42, (1997)

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New plasmid-mediated fluoroquinolone efflux pump, QepA, found in an Escherichia coli clinical isolate. Kunikazu Yamane et al Antimicrob. Agents Chemother. 51, 3354-60, (2007)

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Micelle-induced multiple performance improvement of fluorescent probes for H2S detection. Tian H, Qian J, Bai H, et al. Anal. Chim. Acta 768, 136-42, (2013)

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Disclosure of a structural milieu for the proximity ligation reveals the elusive nature of an active chromatin hub. Gavrilov AA, Gushchanskaya ES, Strelkova O, et al. Nucleic Acids Res. 41(6), 3563-75, (2013)

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Novel cationic lipophilic peptides for oligodeoxynucleotide delivery. Enoch Chan et al Bioorg. Med. Chem. 15, 4091-7, (2007)

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Comparative static curing versus dynamic curing on tablet coating structures. Gendre C, Genty M, Fayard B, et al. Int. J. Pharm. 453(2), 448-53, (2013)

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Incorporation of polyamidoamine sweeping and electrokinetic supercharging for in-line DNA fragment preconcentration. Tian J, Qiao J, Gao J, et al. J. Chromatogr. A 1280, 112-6, (2013)

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Loop sequence dictates the secondary structure of a human membrane protein hairpin. Nadeau VG and Deber CM Biochemistry 52(14), 2419-26, (2013)

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Role of AbeS, a novel efflux pump of the SMR family of transporters, in resistance to antimicrobial agents in Acinetobacter baumannii. Vijaya Bharathi Srinivasan et al Antimicrob. Agents Chemother. 53, 5312-6, (2009)

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Prediction of micelle/water and liposome/water partition coefficients based on molecular dynamics simulations, COSMO-RS, and COSMOmic. Ingram T, Storm S, Kloss L, et al. Langmuir 29(11), 3527-37, (2013)

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Reduced biocide susceptibility in Candida albicans biofilms. Jeniel E Nett et al Antimicrob. Agents Chemother. 52, 3411-3, (2008)

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LmrS is a multidrug efflux pump of the major facilitator superfamily from Staphylococcus aureus. Jody L Floyd et al Antimicrob. Agents Chemother. 54, 5406-12, (2010)

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Comparison of complexes formed by a crustacean and a vertebrate trypsin with bovine pancreatic trypsin inhibitor - the key to achieving extreme stability? Molnár T, Vörös J, Szeder B, et al. FEBS J. 280(22), 5750-63, (2013)

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Cloning, nucleotide sequencing, and analysis of the AcrAB-TolC efflux pump of Enterobacter cloacae and determination of its involvement in antibiotic resistance in a clinical isolate. Astrid Pérez et al Antimicrob. Agents Chemother. 51, 3247-53, (2007)

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Anti-inflammatory activity of verminoside from Kigelia africana and evaluation of cutaneous irritation in cell cultures and reconstituted human epidermis. Patrizia Picerno et al J. Nat. Prod. 68, 1610-4, (2005)

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Substrate competition studies using whole-cell accumulation assays with the major tripartite multidrug efflux pumps of Escherichia coli. Christopher A Elkins et al Antimicrob. Agents Chemother. 51, 923-9, (2007)

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Mechanism of electrolyte-induced brightening in single-wall carbon nanotubes. Duque JG, Oudjedi L, Crochet JJ, et al. J. Am. Chem. Soc. 135(9), 3379-82, (2013)

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The role of amphiphilicity and negative charge in glycoprotein 41 interactions in the hydrophobic pocket. Miriam Gochin et al J. Med. Chem. 52, 4338-44, (2009)

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Fluorescent labeling of proteins with nile red and 2-methoxy-2,4-diphenyl-3(2H)-furanone: physicochemical basis and application to the rapid staining of sodium dodecyl sulfate polyacrylamide gels and Western blots. Daban JR Electrophoresis 22(5), 874-80, (2001)

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Decellularized feeders: an optimized method for culturing pluripotent cells. Lim ML, Jungebluth P, Sjöqvist S, et al. Stem Cells Transl. Med. 2(12), 975-82, (2013)

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Ion pair hollow fiber liquid-liquid-liquid microextraction combined with capillary electrophoresis-ultraviolet detection for the determination of thyroid hormones in human serum. Li P, Hu B, He M, et al. J. Chromatogr. A 1356, 23-31, (2014)

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Antigen retrieval in cells and tissues: enhancement with sodium dodecyl sulfate. Robinson JM and Vandré DD Histochem. Cell Biol. 116(2), 119-30, (2001)

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Non-targeted identification of prions and amyloid-forming proteins from yeast and mammalian cells. Kryndushkin D, Pripuzova N, Burnett BG, et al. J. Biol. Chem. 288(38), 27100-11, (2013)

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Interaction between zwitterionic surface activity ionic liquid and anionic surfactant: Na(+)-driven wormlike micelles. Wang X, Wang R, Zheng Y, et al. J. Phys. Chem. B 117(6), 1886-95, (2013)

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Physicochemical and antibacterial properties of surfactant mixtures with quaternized chitosan microgels. Richardson KE, Xue Z, Huang Y, et al. Carbohydr. Polym. 93(2), 709-17, (2013)

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Evaluation of the potential for diacetylenes as reporter molecules in 3D micelle gel dosimetry. Nasr AT, Olding T, Schreiner LJ, et al. Phys. Med. Biol. 58(4), 787-805, (2013)

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The detection of enzyme activity following sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Bischoff KM, Shi L, and Kennelly PJ Anal. Biochem. 260(1), 1-17, (1998)

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Does CA125 binding to mesothelin impact the detection of malignant mesothelioma? Creaney J, Dick IM, Dare H, et al. Lung Cancer 80(1), 39-44, (2013)

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Influence of membrane structure on the preparation of colloidal lipid dispersions by premix membrane emulsification. Joseph S and Bunjes H Int. J. Pharm. 446(1-2), 59-62, (2013)

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Structural determinants of Tau aggregation inhibitor potency. Schafer KN, Cisek K, Huseby CJ, et al. J. Biol. Chem. 288(45), 32599-611, (2013)

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Docetaxel-loaded polylactic acid-co-glycolic acid nanoparticles: formulation, physicochemical characterization and cytotoxicity studies. Pradhan R, Poudel BK, Ramasamy T, et al. J. Nanosci. Nanotechnol. 13(8), 5948-56, (2013)

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Interface-induced disassembly of a self-assembled two-component nanoparticle system. Gao Y, Duc le T, Ali A, et al. Langmuir 29(11), 3654-61, (2013)

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Bicephalic amphiphile architecture affects antibacterial activity. Jade E LaDow et al Eur. J. Med. Chem. 46, 4219-26, (2011)

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Vesicles and reverse vesicles of an ionic liquid in ionic liquids. Rao KS, So S, and Kumar A Chem. Commun. (Camb.) 49(73), 8111-3, (2013)

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Molecular dynamics of the interaction of anionic surfactants with liposomes. Chen C, Jiang C, and Tripp CP Colloids Surf. B Biointerfaces 105, 173-9, (2013)

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In vitro reconstitution of complexes between pro-matrix metalloproteinase-9 and the proteoglycans serglycin and versican. Malla N, Berg E, Theocharis AD, et al. FEBS J. 280(12), 2870-87, (2013)

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Molecular weight standards for calibration of gel filtration and sodium dodecyl sulfate-polyacrylamide gel electrophoresis: ferritin and apoferritin. de Haen, C. Anal. Biochem. 16, 235, (1987)

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Exposure of RML scrapie agent to a sodium percarbonate-based product and sodium dodecyl sulfate renders PrPSc protease sensitive but does not eliminate infectivity. Smith JD, Nicholson EM, Foster GH, et al. BMC Vet. Res. 9, 8, (2013)

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Shotgun proteomic analysis of two Bartonella quintana strains. Fabietti A, Gaspari M, Krishnan S, et al. Proteomics 13(8), 1375-8, (2013)

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Sequence homology analysis of proteins by chemical cleavages: using a mono and two dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Han KK, Richard C, Zhang GY, et al. Int. J. Biochem. 18(12), 1073-82, (1986)

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Effects of buffered vinegar and sodium dodecyl sulfate plus levulinic acid on Salmonella Typhimurium survival, shelf-life, and sensory characteristics of ground beef patties. Stelzleni AM, Ponrajan A, and Harrison MA Meat Science 95(1), 1-7, (2013)

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Different stratum corneum lipid liposomes as models to evaluate the effect of the sodium dodecyl sulfate. López O, Cócera M, de la Maza A, et al. Biochim. Biophys. Acta 1508(1-2), 196-209, (2000)

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Charged surfactants induce a non-fibrillar aggregation pathway of amyloid-beta peptide. Loureiro JA, Rocha S, and Pereira Mdo C J. Pept. Sci. 19(9), 581-7, (2013)

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Performance of different C18 columns in reversed-phase liquid chromatography with hydro-organic and micellar-organic mobile phases. Ruiz-Angel MJ, Pous-Torres S, Carda-Broch S, et al. J. Chromatogr. A 1344, 76-82, (2014)

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Effect of low concentration sodium dodecyl sulfate on the electromigration of palonosetron hydrochloride stereoisomers in micellar electrokinetic chromatography. Hu SQ, Wang GX, Guo WB, et al. J. Chromatogr. A 1342, 86-91, (2014)

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Epitope scanning indicates structural differences in brain-derived monomeric and aggregated mutant prion proteins related to genetic prion diseases. Tapella L, Stravalaci M, Bastone A, et al. Biochem. J. 454(3), 417-25, (2013)

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Formulation and in vitro and in vivo evaluation of film-coated montelukast sodium tablets using Opadry® yellow 20A82938 on an industrial scale. Zaid AN, Natour S, Qaddomi A, et al. Drug Des. Devel. Ther. 7, 83-91, (2013)

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Micellar-enhanced ultrafiltration of copper ions using sodium dodecyl sulfate and its mixture with Brij 35, Tween 80 and Triton X-100. Zhao B, Li R, Zhong J, et al. Water Sci. Technol. 67(10), 2154-9, (2013)

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Role of sol with iron oxyhydroxide/sodium dodecyl sulfate composites on Fenton oxidation of sorbed phenanthrene in sand. Park JY and Kim JH J. Environ. Manage. 126, 72-8, (2013)

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Use of surface plasmon resonance to study the adsorption of detergents on poly(dimethylsiloxane) surfaces. Gaspar A, Kecskemeti A, and Gomez FA Electrophoresis 34(8), 1249-52, (2013)

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Preparation and evaluation of Zr-beta-FeOOH for efficient arsenic removal. Sun X, Hu C, and Qu J J. Environ. Sci. (China) 25(4), 815-22, (2013)

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Direct real-time quantitative PCR for measurement of host-cell residual DNA in therapeutic proteins. Peper G, Fankhauser A, Merlin T, et al. J. Pharm. Biomed. Anal. 100, 123-30, (2014)

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Effect of surfactant addition on removal of microbubbles using ultrasound. Kobayashi D, Hayashida Y, Sano K, et al. Ultrasonics 54(6), 1425-9, (2014)

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Structural elucidation of transmembrane transporter protein bilitranslocase: conformational analysis of the second transmembrane region TM2 by molecular dynamics and NMR spectroscopy. Roy Choudhury A, Perdih A, Zuperl S, et al. Biochim. Biophys. Acta 1828(11), 2609-19, (2013)

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Efficacy of sanitizers in reducing Salmonella on pecan nutmeats during cracking and shelling. Beuchat LR, Mann DA, and Alali WQ J. Food Prot. 76(5), 770-8, (2013)

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Concentration-dependent antagonistic persuasion of SDS and naphthalene derivatives on the fibrillation of stem bromelain. Qadeer A, Ahmad E, Zaman M, et al. Arch. Biochem. Biophys. 540(1-2), 101-16, (2013)

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Influences of perfluorooctanoic acid on the aggregation of multi-walled carbon nanotubes. Li C, Schäffer A, Vereecken H, et al. J. Environ. Sci. (China) 25(3), 466-72, (2013)

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Amphiphilic suramin dissolves Matrigel, causing an 'inhibition' artefact within in vitro angiogenesis assays. Prigozhina NL, Heisel AJ, Seldeen JR, et al. Int. J. Exp. Pathol. 94(6), 412-7, (2013)

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Biodegradation of anionic surfactant, sodium dodecyl sulphate by Pseudomonas aeruginosa MTCC 10311. Ambily PS and Jisha MS J. Environ Biol. 33(4), 717-20, (2012)

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Sodium dodecyl sulphate, a strong inducer of thermostable glucanhydrolase secretion from a derepressed mutant strain of Bacillus alcalophilus GCBNA-4. Shamim N, Ali S, and Ul-Haq I Appl. Biochem. Biotechnol. 169(8), 2467-77, (2013)

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Rgn gene is required for gut cell homeostasis after ingestion of sodium dodecyl sulfate in Drosophila. Pan J and Jin LH Gene 549(1), 141-8, (2014)

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Nanofiltration and Fenton's process over iron shavings for surfactants removal. Martins RC, Nunes M, Gando-Ferreira LM, et al. Environ. Technol. 35(17-20), 2380-8, (2014)

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Effect of anionic-nonionic-mixed surfactant micelles on solubilization of PAHs. Shi Z, Chen J, and Yin X J. Air Waste Manag. Assoc. 63(6), 694-701, (2013)

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Effects of sodium dodecyl sulphate on enhancement of lipoxygenase activity of hemoglobin. Ezebuo FC, Eze SO, and Chilaka FC Indian J. Exp. Biol. 50(12), 847-52, (2012)

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Effect of essential oils, hydrating agents, and ethanol on hair removal efficiency of thioglycolates. Moghimi HR, Jamali B, Farahmand S, et al. J. Cosmet. Dermatol. 12(1), 41-8, (2013)

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A non-destructive genotyping system from a single seed for marker-assisted selection in watermelon. Meru G, McDowell D, Waters V, et al. Genet. Mol. Res. 12(1), 702-9, (2013)

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Tolerance of droplet-digital PCR vs real-time quantitative PCR to inhibitory substances. Dingle TC, Sedlak RH, Cook L, et al. Clin. Chem. 59(11), 1670-2, (2013)

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