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L4777 Sigma

Lipase acrylic resin from Candida antarctica Green Alternative

≥5,000 U/g, recombinant, expressed in Aspergillus niger

Synonym: Novozyme 435

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Properties

Related Categories 3.1.x.x Acting on esters, 3.x.x.x Hydrolases, Analytical and Industrial Enzymes, Application Index, Biochemicals and Reagents,
recombinant   expressed in Aspergillus niger
greener alternative product characteristics   Waste Prevention: Greener alternative product characteristics
Learn more about the Principles of Green Chemistry.
matrix   macroporous acrylic resin
Featured Industry   Diagnostic Assay Manufacturing
greener alternative category   Enabling
storage temp.   2-8°C

Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Application

Immobilized preparation of a thermostable lipase, particularly useful in the synthesis of esters and
amides, and has a broad substrate specificity.

General description

Lipase from (B lipase) Candida antarctica produced by submerged fermentation of a genetically modified Aspergillus niger microorganism and adsorbed on a macroporous resin.

Sigma Life Science is committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for waste prevention when used in biodiesel research. For more information see the article in biofiles.

Packaging

3, 10 g in poly bottle

Price and Availability


Biomedical Applications
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
1

Documents

Certificate of Analysis

Certificate of Origin

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis. These documents are located on the product detail page under Useful Links & Tools. Click on the following link to search for a Certificate of Analysis. Please click the following link to see the details on our Product Dating Information.
How do I get lot-specific information or a Certificate of Analysis?
A Certificate of Analysis is available by lot number and can be obtained through our Advanced Search Option: http://www.sigmaaldrich.com/catalog/AdvancedSearchPage.do
How do I find price and availability?
There are several ways to find pricing and availability for our products.  Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers. 
What solvents are most compatible with Product L4777, Lipase acrylic resin?
This resin works well in solvent-free reactions, as well as in inert water immiscible solvents such as petroleum ether, n-hexane and n-heptane. If the reaction being catalyzed is hydrolysis of an ester, addition of water to the reaction mix is recommended. For other reactions water is likely not required, and in some cases would actually be detrimental.
What is the substrate specificity of Product L4777, Lipase acrylic resin?
Lipase acrylic resin has a broad substrate specificity. It promotes reactions between a wide range of primary and secondary alcohols and carboxylic acids.
What are the components of Product L4777, Lipase acrylic resin, and what are the optimal recommended conditions?
This product consists of a thermostable lipase enzyme immobilized on a macroporous acrylic bead resin. The bead-shaped particles have a diameter in the range of 0.3-0.9 mm. The resin will be compatible with stearyl alcohol at temperatures of 40-60°C, which is the normal operating temperature of the enzyme. This immobilized enzyme can be used in hexane with no adverse effects.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS. To access the shipping information for this material, use the link on the product detail page for the product, or search here. 
My question is not addressed here, how can I contact Technical Service for assistance?
Use the option to the right to "Ask a Question" by email of a Technical Service Scientist.
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Protocols & Articles

Related Content

Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Molecular biology

Peer-Reviewed Papers
15

References

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