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L4889 Sigma

Lectin from Ulex europaeus (gorse, furze)

TRITC conjugate, lyophilized powder

Synonym: Ulex europaeus agglutinin, UEA-I

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Properties

Related Categories Ulex europaeus (Gorse, furze), Biochemicals and Reagents, Fluorescent Conjugates, Fluorescent Probes, Labels, Particles and Stains, Glycan Recognizing Proteins,
form   lyophilized powder
potency   <10 μg/mL agglutination activity
composition   Protein, ~10% Lowry
extent of labeling   4-8 mol TRITC per mol lectin
conjugate   TRITC conjugate
storage temp.   −20°C

Description

Analysis Note

Agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, of a 1 mg/ml solution. This activity is the lowest concentration to agglutinate a 2% suspension of human blood group O erythrocytes after 1 hour incubation at 25 °C.

Biochem/physiol Actions

Ulex europaeus agglutinin has anti-H blood group specificity. There are two types of lectin: UEA I has an affinity for L-fucose and UEA II has an affinity for N,N′-diacetylchitobiose. The mol. wt. of UEA I was initially found to be 170,000 daltons. Later reports indicate that UEA I may form aggregates at neutral and basic pH and that the correct mol. wt. is 68,000 daltons.

Linkage

Conjugates are prepared from affinity purified lectin.

Packaging

Package size based on protein content.

Physical form

Contains potassium phosphate buffer salts and sodium chloride

Price and Availability


Amplified Detection

Amicon Pro Purification System
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Protocols & Articles
Peer-Reviewed Papers
15

References

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