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L4899 Sigma

Luciferase from Luciola mingrelica

≥85% (SDS-PAGE), recombinant, expressed in E. coli, buffered aqueous glycerol solution

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Properties

Related Categories 1.13.x.x Acting on single donors with incorporation of molecular oxygen, 1.x.x.x Oxidoreductases, Analytical and Industrial Enzymes, Biochemicals and Reagents, Chemiluminescent compounds,
recombinant   expressed in E. coli
assay   ≥85% (SDS-PAGE)
form   buffered aqueous glycerol solution
mol wt   monomer mol wt 61,707 Da
shipped in   wet ice
storage temp.   −20°C

Description

Unit Definition

One light unit produces a biometer peak height equivalent to 0.02 μCi of 14C in PPO/POPOP cocktail. Light units measured in 50 μl assay mixture containing 5 pmol ATP and 7.5 nmol luciferin in Tris-glycine buffer, pH 7.6, at 25 °C.

Physical form

Solution in 25 mM Tris acetate, 5 mM MgSO4, 1 mM EDTA, and 50% glycerol

Price and Availability

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
2

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Related Content

Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Molecular biology

Peer-Reviewed Papers
15

References

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