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L5650 Sigma

Lectin from Narcissus pseudonarcissus (daffodil)

lyophilized powder

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Properties

form   lyophilized powder
potency   <1 μg per mL
impurities   salt, essentially free
storage temp.   2-8°C

Description

Analysis Note

Agglutination activity is expressed in μg per mL and is determined from serial dilution in phosphate buffered saline, pH 7.2, of a 1 mg per mL solution.
This activity is the lowest concentration to agglutinate a 2% suspension of trypsinized rabbit erythrocytes after 1 hr incubation at 25 °C.

Biochem/physiol Actions

Narcissus pseudonarcissus lectin (NPA) agglutinates trypsinized rabbit erythrocytes with high efficiency and untreated rabbit erythrocytes less so; human erythrocytes are not agglutinated.1 NPA sugar specificity is directed to terminal and internal α-D-mannosyl residues of glycoconjugates.2

Preparation Note

Purified by affinity chromatography according to Kaku, H. and Goldstein, I.J., Meth. Enzymol., 179, 327 (1989).

Quality

Highly purified

Price and Availability

Safety & Documentation

Safety Information

WGK Germany 
3

Documents

Certificate of Analysis

Protocols & Articles
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