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L5887 Sigma

Lectin from Vicia villosa (hairy vetch)

FITC conjugate, lyophilized powder

Synonym: Vicia villosa agglutinin, VVA

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Properties

form   lyophilized powder
composition   Protein, ~90% biuret
extent of labeling   3-6 mol FITC per mol protein
conjugate   FITC conjugate
storage temp.   −20°C

Description

Analysis Note

Agglutination activity is expressed in μg per mL and is determined from serial dilutions in phosphate buffered saline, pH 6.8, of a 1 mg per mL solution.
This activity is the lowest concentration to agglutinate a 2% suspension of appropriate erythrocytes after 1 hr incubation at 25°C.

Biochem/physiol Actions

VVA has an affinity for N-acetyl-D-galactosamine. There are at least three tetrameric VVA isolectins composed of subunits A and B, designated B4, A2B2, and A4. Isolectin B4 is specific for Tn activated erythrocytes: A4 has anti-A1 blood group specificity and A2B2 has a specificity intermediate between A4 and B4. There is an additional non-blood group specific lectin in seed extracts.
Conjugates are prepared from affinity purified lectin.

Packaging

Package size based on protein content

Physical form

Lyophilized powder containing sodium citrate

Price and Availability

Safety & Documentation

Safety Information

WGK Germany 
3
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