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L6143 Sigma

Lipopolysaccharides from Salmonella enterica serotype typhimurium

BioXtra, suitable for cell culture, γ-irradiated

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Properties

Related Categories Bacteria-derived adjuvants, Cell Biology, Cell Culture, Cell Signaling and Neuroscience, Immune Cell Signaling and Blood,
sterility   γ-irradiated
product line   BioXtra
form   lyophilized powder
purified by   gel-filtration chromatography
impurities   <1% Protein (Lowry)
suitability   suitable for cell culture
storage temp.   2-8°C

Description

Application

Lipopolysaccharides (LPSs) are characteristic components of the cell wall of Gram-negative bacteria. LPS and its lipid A moiety stimulate cells of the innate immune system by the Toll-like receptor 4 (TLR4), a member of the Toll-like receptor protein family, which recognizes common pathogen-associated molecular-patterns (PAMPs).

Lipopolysaccharides from Salmonella enterica serotype typhimurium has been used:
• in mice to elicit the secretion of pro-inflammatory cytokines
• to induce cytokine production in monocytes
• to elicit the activation of NF-κB pathway in mouse dendritic cells.
• to induce inflammatory response in human colon adenocarcinoma Caco-2 cells
• for stimulation of the catfish peripheral blood leukocytes (PBLs) to study estrogen receptor mRNA expression

Packaging

1 mg in glass bottle

Reconstitution

Lipopolysaccharides are supplied as lyophilized, γ-irradiated powders. To reconstitute, add 1 ml sterile balanced salt solution or tissue culture medium to the vial (1 mg) and gently swirl until the powder dissolves. Reconstituted product may be further diluted to desired working concentrations using sterile balanced salt solution or tissue culture medium.

Biochem/physiol Actions

LPS is a major constituent of the cell wall of most gram negative bacteria. It is a highly immunogenic antigen with the ability to enhance immune responses to soluble antigens. LPS also acts as a specific mitogen for bone marrow derived B lymphocytes from mice, rabbits, chickens, cows, hamsters, and humans.

Price and Availability

Safety & Documentation

Safety Information

Symbol 
GHS07  GHS07
Signal word 
Warning
Hazard statements 
Precautionary statements 
RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Protocols

Lipopolysaccharides

Lipopolysaccharides (LPS) are characteristic components of the cell wall of Gram negative bacteria; they are not found in Gram positive bacteria. They are localized in the outer layer of the membrane...
Keywords: Adsorption, Antibiotics, Cell culture, Cell disruption, Chromatography, Culture media, Filtration, Immunology, Ion Exchange, Metabolism, Positron Emission Tomography, Purification, Size-exclusion chromatography, Solvents

Peer-Reviewed Papers
15

References

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