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L6385 Sigma

α-Lactalbumin from bovine milk

For use as a marker in SDS-PAGE

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Properties

Related Categories Biochemicals and Reagents, Casein and Other Milk Proteins, Proteins and Derivatives More...
mol wt   mol wt ~14.2 kDa
packaging   vial of 5 mg
solubility   H2O: soluble10 mg/mL
storage temp.   2-8°C
Gene Information   cow ... LALBA(281894)

Description

Application

α-Lactalbumin from bovine milk is suitable for use:
• as an electrophoresis marker, with a molar mass of approximately 14,200Da
• in a study to investigate selective binding of proteins on charged surface iron oxide nanoparticles via reverse charge parity model
α-Lactalbumin was used in the reduction of the antigenicity of whey proteins by lactic acid fermentation.

Biochem/physiol Actions

Alters the substrate specificity of galactosyltransferase to increase the rate of lactose formation; the complex of galactosyltransferase and α-lactalbumin is called lactose synthase. Site-directed mutagenesis of Asp87 or Asp88 to Ala completely abolishes the strong calcium binding affinity and reduces the stimulation of lactose synthase to <3.5% of the maximal rate.

α-Lactalbumin consists of a single polypeptide chain with 8 cysteines which form disulfide bridges. α-Lactalbumin binds several metal ions, including calcium, which is thought to play a role in the regeneration of native α-lactalbumin from the reduced, denatured form. α-Lactalbumin also has a distinct zinc binding site that is thought to play a role in the binding of the lactose synthase complex. The mature protein consists of 123 amino acid residues (14 kD), and it has a three-dimensional structure with 1.7 Angstrom resolution, demonstrating four a-helices and a triple stranded antiparallel β-sheet.

General description

α-Lactalbumin is a small, globular, whey protein that has been found in all milk studied to date. It is a metalloprotein of approximately 14 kDa produced in the mammary glands.

Price and Availability

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles
Peer-Reviewed Papers
15

References

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