• USA Home
  • L6397 - Concanavalin A from Canavalia ensiformis (Jack bean)

L6397 Sigma

Concanavalin A from Canavalia ensiformis (Jack bean)

peroxidase conjugate, lyophilized powder

Synonym: ConA



Analysis Note

Where reported, agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, containing Ca2+ and Mn2+ of a 1 mg per mL solution. This activity is the lowest concentration to agglutinate a 2% suspension of human erythrocytes after 1 hr incubation at 25 °C.


Detects glycoproteins containing α-D-mannose, α-D-glucose when used with appropriate peroxidase substrate

General Western Blot Protocol:
• Glycoprotein sample size: 500ng
• Lectin Concentration: 0.1ug/ml

  1. Load samples at 500 ng of glycoprotein per lane
    • Run 4-20% Bis-Tris SDS page gel
    • Transfer gel to a PVDF membrane
    • Block membrane for 1 hr at RT with RIPA buffer (R0278 Sigma)
    • Incubate HRP lectin at 0.1ug/ml with RIPA buffer for 2 hours at RT
    • Wash membrane 5 x 5 minutes with 25ml RIPA buffer
    • Detect using chemiluminescent substrate (CPS1-120)

Biochem/physiol Actions

Con A is not blood group specific but has an affinity for terminal α-D-mannosyl and α-D-glucosyl residues. Ca2+ and Mn2+ ions are required for activity. Con A dissociates into dimers at pH 5.6 or below. Between pH 5.8 and pH 7.0, Con A exists as a tetramer; above pH 7.0 higher aggregates are formed. Con A exhibits mitogenic activity which is dependent on its degree of aggregation. Succinylation results in an active dimeric form which remains a dimer above pH 5.6.


Package size based on protein content

Preparation Note

Prepared from peroxidase type VI (P8375), using the method of Wilson and Nakane, which promotes conjugation but prevents the interaction between Con A and peroxidase sugar residues.

Unit Definition

One unit will form 1 mg purpurogallin in 20 sec from pyrogallol at pH 6.0 at 20 °C.

Physical form

Lyophilized powder containing tris-citrate buffer salts and trace calcium and manganese

Price and Availability

Amplified Detection
Safety & Documentation

Safety Information

GHS08  GHS08
Signal word 
Hazard statements 
Precautionary statements 
NONH for all modes of transport
WGK Germany 
Protocols & Articles
Peer-Reviewed Papers


Related Products

related product

Product #


Add to Cart

06693 Timestrip Plus -20 °C

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?