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L6522 Sigma

Monoclonal Anti-Human Lambda Light Chains (Bound and Free) antibody produced in mouse

clone HP-6054, ascites fluid

Synonym: Monoclonal Anti-Human Lambda Light Chains (Bound and Free)

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Properties

Related Categories Alphabetical Index, Antibodies, HU-HZ, Human Lambda Secondary Antibodies and Conjugates, Primary Antibodies,
biological source   mouse
antibody form   ascites fluid
clone   HP-6054, monoclonal
contains   15 mM sodium azide
application(s)   immunohistochemistry: suitable
  immunoprecipitation: suitable
  indirect ELISA: 1:1,000 using using 2 μg/ml of human IgG myeloma containing lambda light chains as the coating protein
  radioimmunoassay: suitable
  western blot: suitable
isotype   IgG2a
conjugate   unconjugated
shipped in   dry ice
storage temp.   −20°C

Description

Immunogen

Purified human IgG myeloma proteins covalently coupled to polyaminostyrene (PAS) microbeads.

Specificity

The antibody is specific for human lambda (λ) light chains. This antibody is reactive with intact IgG (λ), IgA (λ), free lambda light chains and Bence-Jones lambda light chains. It is non-reactive with kappa light chains.

Application

Monoclonal Anti-Human λ Light Chains (Bound and Free) antibody produced in mouse was used as capture antibody in sandwich ELISA of hybridomas of splenocytes and NS0 myeloma cells at a dilution of 1:500.

Biochem/physiol Actions

Immunoglobulins (Ig) are produced by the B cells and play crucial role in host humoral immune response. A typical immunoglobulin consists of two heavy chains and two light chains. The two light chains are either κ or the λ chain. The B cells express one type of light chain during their life time.

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Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

How to Choose a Secondary Antibody

The following information is provided to help you decide which secondary antibody may be best for your particular application.
Keywords: Amplification, Enzyme-linked immunosorbent assay, Flow cytometry, Immobilization, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western blot

Secondary Antibodies, Conjugates and Kits

Secondary antibodies are polyclonal or monoclonal antibodies that bind to primary antibodies or antibody fragments, such as the Fc or Fab regions. They are typically labeled with probes that make the...
Keywords: Absorption, Adsorption, Amplification, Bacterial conjugations, Cancer, Digestions, Electrophoresis, Enzyme-linked immunosorbent assay, Flow cytometry, Gel electrophoresis, Gene expression, Hormones, Immobilization, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunology, Immunoprecipitation, Infrared spectroscopy, Microbiology, Microscopy, Purification, Vitamins, Western blot

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