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L6876 Sigma

Lysozyme from chicken egg white

lyophilized powder, protein ≥90 %, ≥40,000 units/mg protein

Synonym: Mucopeptide N-acetylmuramoylhydrolase, Muramidase

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Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Application

Enzyme breaks down the cell walls of bacteria; used to prepare spheroplasts.

Lysozyme from chicken egg white has been used for the extraction of genomic DNA from bacterial cells. It has been used as an external standard for MALDI-TOF (matrix assisted laser desorption ionization-time of flight) mass analysis.

Other Notes

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

Packaging

1, 5, 10, 25, 100 g in poly bottle

Unit Definition

One unit will produce a ΔA450 of 0.001 per min at pH 6.24 at 25 °C, using a suspension of Micrococcus lysodeikticus as substrate, in a 2.6 mL reaction mixture (1 cm light path).

Biochem/physiol Actions

Lysozyme hydrolyzes β(1→4) linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrin. Gram-positive cells are quite susceptible to this hydrolysis as their cell walls have a high proportion of peptidoglycan. Gram-negative bacteria are less susceptible due to the presence of an outer membrane and a lower proportion of peptidoglycan. However, these cells may be hydrolyzed in the presence of EDTA that chelates metal ions in the outer bacterial membrane.

The enzyme is active over a broad pH range (6.0 to 9.0). At pH 6.2, maximal activity is observed over a wider range of ionic strengths (0.02 to 0.100 M) than at pH 9.2 (0.01 to 0.06 M).

Price and Availability


All labs need water

Biomedical Applications
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
RTECS 
OL5989000

Frequently Asked Questions

How can Product L6876, Lysozyme from chicken egg white, be reconstituted and can stock solutions be prepared?
For the lysis procedure, use a freshly prepared solution of 500,000 units/ml in 10 mM Tris-HCl, pH 8.0. The product is also soluble in water (10 mg/ml) yielding a clear to slightly hazy colorless solution. Aqueous solutions should be stable for at least one month when stored between 2–8 °C. To view this information on the L6876 data sheet click on the following link:   http://www.sigmaaldrich.com/etc/medialib/docs/Sigma/Datasheet/7/l6876dat.Par.0001.File.tmp/l6876dat.pdf
How is the activity of Product L6876, Lysozyme from chicken egg white, measured?
The method of enzymatic analysis which is utilized by Sigma to measure the activity of this enzyme can be viewed by clicking on the following link: http://www.sigmaaldrich.com/etc/medialib/docs/Sigma/Enzyme_Assay/lysozyme6.Par.0001.File.dat/lysozyme6.pdf
Can Product L6876, Lysozyme from chicken egg white, be inactivated by heat?
Solutions of Lysozyme may be inactivated by boiling for 40 seconds at 100°C at an alkaline pH.
What is the amino acid sequence of Product L6876, Lysozyme from chicken egg white?
Lysozyme is a single chain polypeptide of 129 amino acids. The amino acid sequence of chicken egg white Lysozyme is described in the Journal of Biological Chemistry, 238, 2698-2707 (1963).
Under what conditions can Product L6876, Lysozyme from chicken egg white, be used to lyse E. coli?
The following procedure is for the lysis of E. coli. It may be used as a guideline for other species. 1. Incubate E. coli (strain ATCC 37017) bearing the pBR322 plasmid overnight in Terrific Broth (Product No. T 0918) with 25 μg/ml tetracycline (Product No T 3383) and 25 μg/ml ampicillin (Product No. A 9518). 2. Centrifuge 1-2 ml samples of the overnight culture. 3. Resuspend the pellets in 350 μl of STET buffer (10 mM Tris-HCl, pH 8.0, with 0.1 M NaCl, 1 mM EDTA, and 5% [w/v] TRITON® X-100). 4. Add 25 μl of a freshly prepared lysozyme solution (500,000 units/ml in 10 mM Tris-HCl, pH 8.0). 5. Mix by vortexing for 3 seconds. 6. Incubate the lysis mixture for 30 minutes at 37 °C 7. After incubation, place the tube containing the lysis mixture in a boiling water bath for exactly 40 seconds. 8. Centrifuge the lysis mixture at 14,000 x g. 9. Remove the pellet (cell debris) from the tube using a sterile toothpick. 10. Plasmid DNA from the supernatant may then be purified and analyzed. To view this information of the L6876 data sheet click on the following link: http://www.sigmaaldrich.com/etc/medialib/docs/Sigma/Datasheet/7/l6876dat.Par.0001.File.tmp/l6876dat.pdf
Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis. These documents are located on the product detail page under Useful Links & Tools. Click on the following link to search for a Certificate of Analysis. Please click the following link to see the details on our Product Dating Information.
How do I get lot-specific information or a Certificate of Analysis?
A Certificate of Analysis is available by lot number and can be obtained through our Advanced Search Option: http://www.sigmaaldrich.com/catalog/AdvancedSearchPage.do
How do I find price and availability?
There are several ways to find pricing and availability for our products.  Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers. 
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS. To access the shipping information for this material, use the link on the product detail page for the product, or search here. 
My question is not addressed here, how can I contact Technical Service for assistance?
Use the option to the right to "Ask a Question" by email of a Technical Service Scientist.
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Protocols & Articles

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Protocols

Enzymatic Assay of Lysozyme (EC 3.2.1.17)

This procedure may be used for Lysozyme products. The enzymatic rate determination (A450, Light path = 1 cm) is based on the following reaction:

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Peer-Reviewed Papers
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References

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