|Related Categories||3.2.x.x Glycosidases, 3.x.x.x Hydrolases, Application Index, Bacterial, Biochemicals and Reagents,|
|mol wt||single-chain mol wt 14.3 kDa|
|Gene Information||chicken ... LYZ(396218)|
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Enzyme breaks down the cell walls of bacteria; used to prepare spheroplasts.
One unit will produce a ΔA450 of 0.001 per min at pH 6.24 at 25 °C, using a suspension of Micrococcus lysodeikticus as substrate, in a 2.6 mL reaction mixture (1 cm light path).
Dialyzed and lyophilized, containing buffer salts as sodium acetate and sodium chloride
Lysozyme hydrolyzes β(1→4) linkages between N-acetylmuramic acid and N-acetyl-
The enzyme is active over a broad pH range (6.0 to 9.0). At pH 6.2, maximal activity is observed over a wider range of ionic strengths (0.02 to 0.100 M) than at pH 9.2 (0.01 to 0.06 M).
Certificate of Analysis
Certificate of Origin
1. OBJECTIVE The objective of this procedure is to standardize a method for the enzymatic assay of Lysozyme.
From our library of Related Content, Sigma-Aldrich presents Enzyme Explorer: the most comprehensive source of enzymes, substrates, activators, & inhibitors.
Keywords: Cell culture, Cell disruption, Cell signaling, Diagnostic, Digestions, Drug discovery, Functional genomics, Gene expression, Genomics, Metabolic Pathways, Molecular biology, Neuroscience, Proteomics
Inelastic X-ray scattering studies of the short-time collective vibrational motions in hydrated lysozyme powders and their possible relation to enzymatic function. Wang Z, Bertrand CE, Chiang WS, et al. J. Phys. Chem. B 117(4), 1186-95, (2013)
A novel transition-state analogue for lysozyme, 4-O-β-tri-N-acetylchitotriosyl moranoline, provided evidence supporting the covalent glycosyl-enzyme intermediate. Ogata M, Umemoto N, Ohnuma T, et al. J. Biol. Chem. 288(9), 6072-82, (2013)
[Pharmacological correction of nonspecific resistance and production of proinflammatory cytokines during chronic intoxication with organophosphorus compound VX]. Zabrodskiĭ PF and Grishin VA Eksp. Klin. Farmakol. 75(11), 19-21, (2012)
Differential analysis of bactericidal systems of blood serum with recombinant luminescent Escherichia coli and Bacillus subtilis strains. Deryabin DG, Karimov IF, Manukhov IV, et al. Bull. Exp. Biol. Med. 154(1), 59-63, (2012)
Investigation on the interaction between cyclophosphamide and lysozyme in the presence of three different kind of cyclodextrins: determination of the binding mechanism by spectroscopic and molecular modeling techniques. Mansouri M, Pirouzi M, Saberi MR, et al. Molecules 18(1), 789-813, (2013)
Localization and orientation of heavy-atom cluster compounds in protein crystals using molecular replacement. Dahms SO, Kuester M, Streb C, et al. Acta Crystallogr. D, Biol. Crystallogr. 69(Pt 2), 284-97, (2013)
Inheritance of the lysozyme inhibitor Ivy was an important evolutionary step by Yersinia pestis to avoid the host innate immune response. Derbise A, Pierre F, Merchez M, et al. J. Infect. Dis. 207(10), 1535-43, (2013)
Physical entrapment of hyaluronic acid during synthesis results in extended release from model hydrogel and silicone hydrogel contact lens materials. Weeks A, Subbaraman LN, Jones L, et al. Eye Contact Lens 39(2), 179-85, (2013)
A universal, high recovery assay for protein quantitation through temperature programmed liquid chromatography (TPLC). Orton DJ and Doucette AA J. Chromatogr. B. Analyt. Technol. Biomed. Life Sci. 921-922, 75-80, (2013)
Expression of recombinant human lysozyme-tachyplesin I (hLYZ-TP I) in Pichia pastoris and analysis of antibacterial activity. Gao Y, Zhao HL, Feng X, et al. Biomed. Environ. Sci. 26(4), 319-22, (2013)
[The approaches to realization of the therapeutic action of gas-air carbonate baths and lysozyme inhalation during their combined application for the treatment of patients presenting with chronic obstructive pulmonary disease]. Aĭrapetova NS, Uianaeva MA, and Pershin SB Vopr. Kurortol. Fizioter. Lech. Fiz. Kult. (2), 8-13, (2013)
Balance between early life tolerance and sensitization in allergy: dependence on the timing and intensity of prenatal and postnatal allergen exposure of the mother. Ana Elisa Fusaro et al Immunology 128, e541-50, (2009)
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