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L7001 Sigma

Lysozyme from chicken egg white

lyophilized powder, ~50,000 units/mg protein (E1%/282)

Synonym: Mucopeptide N-acetylmuramoylhydrolase, Muramidase

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Properties

Related Categories 3.2.x.x Glycosidases, 3.x.x.x Hydrolases, Application Index, Bacterial, Biochemicals and Reagents,
type   Grade III
form   lyophilized powder
mol wt   single-chain mol wt 14.3 kDa
composition   Protein, ~85%
storage temp.   −20°C
Gene Information   chicken ... LYZ(396218)

Description

Application

Enzyme breaks down the cell walls of bacteria; used to prepare spheroplasts.

Other Notes

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

Preparation Note

3× Crystallized

Unit Definition

One unit will produce a ΔA450 of 0.001 per min at pH 6.24 at 25 °C, using a suspension of Micrococcus lysodeikticus as substrate, in a 2.6 mL reaction mixture (1 cm light path).

Physical form

Lyophilized powder containing sodium acetate buffer salts and sodium chloride

Biochem/physiol Actions

Lysozyme hydrolyzes β(1→4) linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrin. Gram-positive cells are quite susceptible to this hydrolysis as their cell walls have a high proportion of peptidoglycan. Gram-negative bacteria are less susceptible due to the presence of an outer membrane and a lower proportion of peptidoglycan. However, these cells may be hydrolyzed in the presence of EDTA that chelates metal ions in the outer bacterial membrane.

The enzyme is active over a broad pH range (6.0 to 9.0). At pH 6.2, maximal activity is observed over a wider range of ionic strengths (0.02 to 0.100 M) than at pH 9.2 (0.01 to 0.06 M).

Price and Availability

Safety & Documentation

Safety Information

WGK Germany 
3
RTECS 
OL5989000
Protocols & Articles

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Peer-Reviewed Papers
15

References

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