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L7009 Sigma

Prolactin human

recombinant, expressed in E. coli, >97% (SDS-PAGE), lyophilized powder, cell culture tested

Synonym: LTH, Lactogenic Hormone, Luteotropic Hormone, PRL

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Properties

Related Categories Cell Biology, Cell Signaling and Neuroscience, Cytokines, Growth Factors and Hormones, Hormones, Luteotropic Hormone (Prolactin),
recombinant   expressed in E. coli
description   200 amino acid residue methionyl form of mature, recombinant human prolactin (amino acid residues 29-227).
assay   >97% (SDS-PAGE)
form   lyophilized powder
mol wt   predicted mol wt ~24 kDa
suitability   cell culture tested
storage temp.   −20°C
Gene Information   human ... PRL(5617)

Description

Analysis Note

Biological activity is measured in a cell proliferation assay using the rat lymphoma, Nb2-11.

Reconstitution

It is recommended that sterile 4 mM HCl containing 1 mg/mL bovine serum albumin be added to the vial to prepare a stock solution of no less than 10 μg/mL.

Physical form

Lyophilized from a 0.2 μm filtered solution in 10 mM phosphate and 50 mM NaCl, pH 8.0.

Biochem/physiol Actions

Prolactin is a neuroendocrine hormone. The prolactin receptor is a transmembrane glycoprotein that belongs to the cytokine hematopoietic receptor family. A large number of cells and organs express the receptor, including B cells, T cells, macrophages, monocytes and neutrophils. Prolactin signal transduction involves the JAK/STAT families and the src kinase family. Induces lactation; inhibits secretion of gonadotropins; release is inhibited by dopamine.

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Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
2

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles
Peer-Reviewed Papers
15

References

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L4021 Prolactin human, recombinant, expressed in E. coli, lyophilized powder, BioReagent, cell culture tested, >97% (SDS-PAGE)

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